申请人:3M INNOVATIVE PROPERTIES COMPANY
公开号:US11414641B2
公开(公告)日:2022-08-16
A thin film culture device for detecting aerobic bacteria in a sample is provided. The culture device comprises a self-supporting substrate sheet having a first major surface and a second major surface; a cover sheet attached to the substrate sheet, a sample-receiving zone disposed between the substrate sheet and the cover sheet, a first layer comprising a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to a portion of the sample-receiving zone; and a plurality of indicator agents disposed in at least one layer adhered to the substrate sheet or the cover sheet. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, an indicator agent for detecting a phosphatase enzyme activity, and a redox indicator. A method of using the culture device is also provided.
提供了一种用于检测样品中需氧细菌的薄膜培养装置。该培养装置包括:具有第一大表面和第二大表面的自支撑基片;连接到基片上的盖片,设置在基片和盖片之间的样品接收区,粘附到样品接收区部分的第一层,包括基本干燥、冷水可溶的第一水凝胶形成组合物;以及设置在粘附到基片或盖片上的至少一层中的多种指示剂。多种指示剂包括三种用于检测不同糖苷酶酶活的指示剂、一种用于检测烷基酯酶酶活的指示剂、一种用于检测磷酸酶酶活的指示剂和一种氧化还原指示剂。还提供了一种使用培养装置的方法。