PC-Maleimide-OH | 1408057-90-8

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: PC-Maleimide-OH
• 英文别名: N-[2-(2,5-dioxopyrrol-1-yl)ethyl]-4-[4-(1-hydroxyethyl)-2-methoxy-5-nitrophenoxy]butanamide
• CAS: 1408057-90-8
• 化学式: C₁₉H₂₃N₃O₈
• 分子量: 421.407
• InChiKey: JSFQSJFHWJOHMW-UHFFFAOYSA-N

物化性质

• 沸点：
  709.2±60.0 °C(Predicted)
• 密度：
  1.355±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  0.1
• 重原子数：
  30
• 可旋转键数：
  10
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.42
• 拓扑面积：
  151
• 氢给体数：
  2
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  N,N'-二琥珀酰亚胺基碳酸酯 、 PC-Maleimide-OH 在 三乙胺 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 18.0h， 以70%的产率得到PC Mal-NHS carbonate ester
  参考文献：
  名称：

  Photocleavable DNA Barcode–Antibody Conjugates Allow Sensitive and Multiplexed Protein Analysis in Single Cells

  摘要：

  DNA barcoding is an attractive technology, as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here we describe the development and validation of a photocleavable DNA barcode-antibody conjugate method for rapid, quantitative, and multiplexed detection of proteins in single live cells. Following target binding, this method allows DNA barcodes to be photoreleased in solution, enabling easy isolation, amplification, and readout As a proof of principle, wedemonstrate sensitive and multiplexed detection of protein biomarkers in a variety of cancer cells.

  DOI：

  10.1021/ja307689w
• 作为产物：
  描述：

  N-(2-氨基乙基)马来酰亚胺三氟乙酸盐 、 4-(4-(1-羟基乙基)-2-甲氧基-5-硝基苯氧基)丁酸 在 O-(1H-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate 、 三乙胺 作用下， 以 二氯甲烷 为溶剂， 反应 18.33h， 以60%的产率得到PC-Maleimide-OH
  参考文献：
  名称：

  Photocleavable DNA Barcode–Antibody Conjugates Allow Sensitive and Multiplexed Protein Analysis in Single Cells

  摘要：

  DNA barcoding is an attractive technology, as it allows sensitive and multiplexed target analysis. However, DNA barcoding of cellular proteins remains challenging, primarily because barcode amplification and readout techniques are often incompatible with the cellular microenvironment. Here we describe the development and validation of a photocleavable DNA barcode-antibody conjugate method for rapid, quantitative, and multiplexed detection of proteins in single live cells. Following target binding, this method allows DNA barcodes to be photoreleased in solution, enabling easy isolation, amplification, and readout As a proof of principle, wedemonstrate sensitive and multiplexed detection of protein biomarkers in a variety of cancer cells.

  DOI：

  10.1021/ja307689w

文献信息

• DEVICES AND METHODS FOR SAMPLE ANALYSIS
  申请人：Abbott Laboratories

  公开号：US20180095067A1

  公开（公告）日：2018-04-05

  Integrated microfluidic and analyte detection devices are disclosed, along with methods of detecting target analytes. Digital microfluidic and analyte detection devices include a first substrate and a second substrate aligned generally parallel to each other to define a gap therebetween, the first substrate including a plurality of electrodes to generate electrical actuation forces on a liquid droplet disposed in the gap; at least one reagent disposed on at least one of the first substrate or the second substrate and configured to be carried by the liquid droplet; and an analyte detection device in fluid communication with the gap, wherein the plurality of electrodes are configured to move the liquid droplet towards the analyte detection device.
• [EN] DEVICES AND METHODS FOR SAMPLE ANALYSIS<br/>[FR] DISPOSITIFS ET PROCÉDÉS D'ANALYSE D'ÉCHANTILLON
  申请人：ABBOTT LAB

  公开号：WO2016161402A1

  公开（公告）日：2016-10-06

  Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.