The present invention relates to a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises extracting the proanthocyanidin oligomers from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by solid-liquid extraction using methyl acetate as a liquid phase; a process for purifying dimeric to pentameric proanthocyanidin oligomers which comprises pretreating with an enzyme for hydrolysis raw materials containing the proanthocyanidin oligomers, crude purification products therefrom, or a solution containing one of these; and a process for purifying dimeric to pentameric proanthocyanidin oligomers with a uniform polymerization degree which comprises separating and purifying the proanthocyanidin oligomers by polymerization degree from raw materials containing the proanthocyanidin oligomers or crude purification products therefrom by normal phase silica gel liquid chromatography using as a mobile phase a single solvent or a mixed solvent of two or more solvents selected from the group consisting of an ester solvent, a ketone solvent, a hydrocarbon solvent, an ether solvent and an alcohol solvent.
本发明涉及一种提纯二聚体至五聚体
原花青素低聚物的工艺,该工艺包括以
醋酸甲酯为液相,通过固液萃取从含有
原花青素低聚物的原料或其粗提纯产物中提取
原花青素低聚物;一种提纯二聚到五聚
原花青素低聚物的工艺,包括用一种
水解酶对含有
原花青素低聚物的原料、其粗提纯产物或含有其中之一的溶液进行预处理;以及一种纯化具有均匀聚合度的二聚体至五聚体
原花青素低聚物的工艺,该 工艺包括通过正相
硅胶液相色谱法从含有
原花青素低聚物或其粗提纯产物的原材 料中按聚合度分离和纯化
原花青素低聚物、
酮类溶剂、烃类溶剂、
醚类溶剂和
醇类溶剂。