A method for the preparation of microvesicles useful as contrast agent in photoacoustic applications; said method being characterised in that it comprises the steps of:
- preparing a graphene water suspension, during which a water solution of graphene and of a first surfactant, which comprises an aromatic structure suited to interact with graphene and reactive functional groups suited to perform a cross-linking step, is subject to the action of ultrasounds;
- emulsion, during which at least one graphene suspension produced in the previous step, a low boiling point perfluorocarbon, a second surfactant, and a biodegradable polymer functionalised with reactive functional groups suited to cause a cross-linking reaction of the biodegradable polymer with the first surfactant present in the graphene suspension are added to and emulsified in water;
- cross-linking, during which the reactive functional groups present in the biodegradable polymer and in the first surfactant cause a cross-linking process with the formation of microvesicles which include the perfluorocarbon.
一种在光声应用中用作造影剂的微囊的制备方法,其特征在于该方法包括以下步骤
- 制备
石墨烯水悬浮液,在此过程中,将
石墨烯和第一种表面活性剂的
水溶液置于超声波作用下;
- 乳化:在乳化过程中,将上一步制备的至少一种
石墨烯悬浮液、一种低沸点
全氟碳化合物、第二种表面活性剂和一种
生物可降解聚合物加入
水中并乳化,该
生物可降解聚合物具有活性官能团,可与
石墨烯悬浮液中的第一种表面活性剂发生交联反应;
- 交联,在此过程中,
生物可降解聚合物和第一种表面活性剂中的活性官能团引起交联反应,形成包含
全氟碳化物的微囊。