4-[(tert-butoxycarbonyl)amino]-1-(4-pyrenylbutyl)pyrrole-2-carboxylic acid | 959127-78-7

• 分子结构分类: 有机化合物 - 苯类化合物 - 芘
• 英文名称: 4-[(tert-butoxycarbonyl)amino]-1-(4-pyrenylbutyl)pyrrole-2-carboxylic acid
• CAS: 959127-78-7
• 化学式: C₃₀H₃₀N₂O₄
• 分子量: 482.579
• InChiKey: ISXXMZKPVYHQCC-UHFFFAOYSA-N

反应信息

• 作为反应物：
  描述：

  4-[(tert-butoxycarbonyl)amino]-1-(4-pyrenylbutyl)pyrrole-2-carboxylic acid 在 盐酸 作用下， 以 乙酸乙酯 为溶剂， 反应 5.0h， 生成
  参考文献：
  名称：

  Detection of CAG repeat DNA sequences by pyrene-functionalized pyrrole-imidazole polyamides

  摘要：

  Five N-methylpyrrole-N-methylimidazole (Py-Im) polyamides possessing a fluorescent pyrene were synthesized by Fmoc solid-phase synthesis using Py/Im monomers and pyrenylbutyl-pyrrole monomer compound 9. The steady state fluorescence of conjugates 1-5 was examined in the presence and absence of (CAG)(12)-containing oligodeoxynucleotides (ODNs) 1 and 2. Of the conjugates, conjugate I showed no background emission around 470 nm in the absence of ODNs, and a clear increase of emission at 475 run was observed upon addition of ODNs I and 2. The emission of conjugate I at 475 nm increased linearly with the concentration of ODN and the number of CAG repeats. The results indicate that conjugate I efficiently forms a pyrene excimer upon binding in the minor groove of DNA. (C) 2007 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2007.07.055
• 作为产物：
  描述：

  在 lithium hydroxide 作用下， 以 四氢呋喃 、 水 为溶剂， 反应 28.0h， 以1.46 g的产率得到4-[(tert-butoxycarbonyl)amino]-1-(4-pyrenylbutyl)pyrrole-2-carboxylic acid
  参考文献：
  名称：

  Detection of CAG repeat DNA sequences by pyrene-functionalized pyrrole-imidazole polyamides

  摘要：

  Five N-methylpyrrole-N-methylimidazole (Py-Im) polyamides possessing a fluorescent pyrene were synthesized by Fmoc solid-phase synthesis using Py/Im monomers and pyrenylbutyl-pyrrole monomer compound 9. The steady state fluorescence of conjugates 1-5 was examined in the presence and absence of (CAG)(12)-containing oligodeoxynucleotides (ODNs) 1 and 2. Of the conjugates, conjugate I showed no background emission around 470 nm in the absence of ODNs, and a clear increase of emission at 475 run was observed upon addition of ODNs I and 2. The emission of conjugate I at 475 nm increased linearly with the concentration of ODN and the number of CAG repeats. The results indicate that conjugate I efficiently forms a pyrene excimer upon binding in the minor groove of DNA. (C) 2007 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmc.2007.07.055