Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
申请人:Schultz Peter G.
公开号:US20090227002A1
公开(公告)日:2009-09-10
This invention provides compositions and methods for generating components of protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Methods for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.