申请人:KABUSHIKI KAISHA YAKULT HONSHA
公开号:EP0115936A1
公开(公告)日:1984-08-15
A chimeric plasmid comprising:
(a) a tetracycline resistant gene deriving from pAMal, one of the plasmids retained by Streptococcus faecalis,
(b) an ampicillin resistantgene deriving from pACYCl77; one of the vectors applicable to Escherichia coli, and
(c) at least one of (i) an area at which repliqation originates deriving from said plasmid pAMal, and (ii) an area at which replication originates deriving from said vector pACYCl77, said tetracycline resistant gene containing only one site at which said chimeric plasmid is recognized and cleaved by the restriction enzyme Ball and said ampicillin resistant gene containing only one site at which said chimeric plasmid is recognized and cleaved by the restriction enzymes Bgll and Pstl. The chimeric plasmid is useful as a vector for conveying a gene into a host cell, usually of a bacterium, and the host can then be employed in biosynthesis of industrial products.
一种嵌合质粒,包括
(a) 源自 pAMal 的抗四环素基因,pAMal 是粪链球菌保留的质粒之一、
(b) 源自 pACYCl77 的抗氨苄西林基因;pACYCl77 是适用于大肠杆菌的载体之一,以及
(c) (i) 源自所述质粒 pAMal 的复制起源区域和 (ii) 源自所述载体 pACYCl77 的复制起源区域中的至少一个,所述抗四环素基因仅包含一个限制性酶 Ball 可识别和裂解所述嵌合质粒的位点,所述抗氨苄西林基因仅包含一个限制性酶 Bgll 和 Pstl 可识别和裂解所述嵌合质粒的位点。这种嵌合质粒可用作载体,将基因导入宿主细胞(通常是细菌),然后利用宿主细胞进行工业产品的生物合成。