2,3,6-trichloro-phenetole | 109345-48-4

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯酚醚
• 英文名称: 2,3,6-trichloro-phenetole
• 英文别名: Aethyl-(2.3.6-trichlor-phenyl)-aether；2,3,6-Trichlor-phenetol；1,2,4-Trichloro-3-ethoxybenzene
• CAS: 109345-48-4
• 化学式: C₈H₇Cl₃O
• 分子量: 225.502
• InChiKey: HULIVERXNCNZGR-UHFFFAOYSA-N

物化性质

• 沸点：
  270.9±35.0 °C(Predicted)
• 密度：
  1.360±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  4.1
• 重原子数：
  12
• 可旋转键数：
  2
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  9.2
• 氢给体数：
  0
• 氢受体数：
  1

反应信息

• 作为反应物：
  描述：

  2,3,6-trichloro-phenetole 、 氨 生成 三氯酚
  参考文献：
  名称：

  Vi-Suppressed Wild StrainSalmonella typhiCultured in High Osmolarity Is Hyperinvasive toward Epithelial Cells and Destructive of Peyer's Patches

  摘要：

  AbstractSalmonella typhi GIFU10007–3 which lost a viaB locus on its chromosome became highly invasive in our previous study. To investigate the phenomenon, we controlled Vi expression in wild strain S. typhi GIFU10007, and studied the invasive phenotype both in vitro and in vivo. When the wild strain of S. typhi was cultured in 300 mm NaCl containing Luria‐Bertani broth (LBH), the expression of Vi antigen was suppressed, but secretion of invasion proteins (SipC, SipB and SipA) was increased. In this condition, wild strain S. typhi became highly invasive toward both epithelial cells and M cells of rat Peyer's patches. When GIFU10007 was cultured under conditions of high osmolarity, the bacteria disrupted Peyer's patches and induced massive bleeding in these structures only 20 min after inoculation into the ileal loop. In contrast, Vi‐encapsulated wild strain GIFU10007 cultured under low osmolarity was not destructive, even after 60 min. To understand the role of the type III secretion system under conditions of high osmolarity, we knocked out the invA and sipC genes of both GIFU10007 and GIFU10007–3. Neither invA nor sipC mutants could invade epithelial cells or M cells in a high osmolarity environment. Our data show that the highly invasive phenotype was only expressed when the wild strain S. typhi was cultured under high osmolarity, which induced a state of Vi suppression, and in the presence of the type III secretion system.

  DOI：

  10.1111/j.1348-0421.2001.tb01283.x
• 作为产物：
  描述：

  碘乙烷 、 alkaline earth salt of/the/ methylsulfuric acid 在 乙醇 作用下， 生成 2,3,6-trichloro-phenetole
  参考文献：
  名称：

  Vi-Suppressed Wild StrainSalmonella typhiCultured in High Osmolarity Is Hyperinvasive toward Epithelial Cells and Destructive of Peyer's Patches

  摘要：

  AbstractSalmonella typhi GIFU10007–3 which lost a viaB locus on its chromosome became highly invasive in our previous study. To investigate the phenomenon, we controlled Vi expression in wild strain S. typhi GIFU10007, and studied the invasive phenotype both in vitro and in vivo. When the wild strain of S. typhi was cultured in 300 mm NaCl containing Luria‐Bertani broth (LBH), the expression of Vi antigen was suppressed, but secretion of invasion proteins (SipC, SipB and SipA) was increased. In this condition, wild strain S. typhi became highly invasive toward both epithelial cells and M cells of rat Peyer's patches. When GIFU10007 was cultured under conditions of high osmolarity, the bacteria disrupted Peyer's patches and induced massive bleeding in these structures only 20 min after inoculation into the ileal loop. In contrast, Vi‐encapsulated wild strain GIFU10007 cultured under low osmolarity was not destructive, even after 60 min. To understand the role of the type III secretion system under conditions of high osmolarity, we knocked out the invA and sipC genes of both GIFU10007 and GIFU10007–3. Neither invA nor sipC mutants could invade epithelial cells or M cells in a high osmolarity environment. Our data show that the highly invasive phenotype was only expressed when the wild strain S. typhi was cultured under high osmolarity, which induced a state of Vi suppression, and in the presence of the type III secretion system.

  DOI：

  10.1111/j.1348-0421.2001.tb01283.x