3-Phosphohistidin | 5789-15-1

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 3-Phosphohistidin
• 英文别名: 3-phosphono-histidine；2-Amino-3-(3-phosphonoimidazol-4-yl)propanoic acid
• CAS: 5789-15-1
• 化学式: C₆H₁₀N₃O₅P
• 分子量: 235.136
• InChiKey: VOHVXLVXSYAFOA-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -4.8
• 重原子数：
  15
• 可旋转键数：
  4
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.33
• 拓扑面积：
  139
• 氢给体数：
  4
• 氢受体数：
  7

安全信息

• 海关编码：
  2933290090

文献信息

• PHOSPHOHISTIDINE ANALOGS
  申请人：Muir Tom W.

  公开号：US20130260398A1

  公开（公告）日：2013-10-03

  The present invention relates to the phosphohistidine analogs of the present invention which of the formula (I) and the hapten containing the residue of same. It also relates to the hapten conjugated to a carrier molecule and the isolated antibodies raised against the immunogens, said antibodies recognizing polypeptide containing a phosphorylated histidine or the phosphotriazole residue but it does not recognize an amino acid or polypeptide that is not phosphorylated or a polypeptide which is phosphorylated on amino acids other than histidine but not on histidine.

  本发明涉及公式（I）的磷酸组氨酸类似物以及含有同样残基的半抗原。本发明还涉及与载体分子结合的半抗原和针对免疫原的分离抗体，所述抗体识别含有磷酸化组氨酸或磷酸三唑残基的多肽，但不识别未磷酸化的氨基酸或多肽，也不识别在组氨酸以外的氨基酸上磷酸化但未在组氨酸上磷酸化的多肽。
• Compositions and methods for analyzing histidine phosphorylation
  申请人：SANOFI

  公开号：EP2778176A1

  公开（公告）日：2014-09-17

  A peptide of the general structure: is provided, wherein X is a non--hydrolyzable pHis analogue and R is H or CH3. Such peptides can be used to produce sequence-independent anti-phosphohistidine antibodies. Also provided is a composition comprising antibodies that specifically bind to said peptide when X is a phosphohistidine (or a non--hydrolyzable pHis analogue) but fail to specifically bind to said peptide when X is histidine.

  一般结构的肽： 其中 X 是不可水解的 pHis 类似物，R 是 H 或 CH3。这种肽可用于生产序列无关的抗磷组氨酸抗体。此外，还提供了一种包含抗体的组合物，当 X 为磷酸组氨酸（或不可水解的 pHis 类似物）时，抗体与所述多肽特异性结合，但当 X 为组氨酸时，抗体不能与所述多肽特异性结合。
• Compositions and methods for detection and isolation of phosphorylated molecules
  申请人：——

  公开号：US20040038306A1

  公开（公告）日：2004-02-26

  The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

  本发明涉及磷酸盐结合化合物，可用于结合、检测和分离磷酸化目标分子，包括随后鉴定与磷酸化目标分子或能够被磷酸化的分子相互作用的目标分子。本发明提供了一种结合溶液，它由磷酸盐结合化合物、酸和金属离子组成，其中金属离子同时与目标分子上暴露的磷酸基团和磷酸盐结合化合物的金属螯合基团相互作用，在磷酸盐结合化合物和磷酸化目标分子之间形成桥梁，形成三元复合物。本发明的结合溶液可用于结合和检测固定化和溶解化的磷酸化目标分子，从复合物混合物中分离磷酸化目标分子，以及协助蛋白质组分析，从而鉴定激酶和磷酸酶底物和酶。
• Phosphoamidase assay
  申请人：Kellner Roland

  公开号：US20050124023A1

  公开（公告）日：2005-06-09

  The present invention relates to the use of compounds and to methods for the qualitative and/or quantitative determination of the activity of phosphoamidases or protein phosphoamidases specific for hydrolyzing phosphoamide (P—N) bonds. N-phosphorylated basic amino acids like phospholysine, phosphoarginine and phosphohistidine.

  本发明涉及化合物的使用以及定性和/或定量测定专门用于水解磷酸酰胺（P-N）键的磷酸酰胺酶或蛋白质磷酸酰胺酶活性的方法。N-磷酸化碱性氨基酸，如磷酰赖氨酸、磷精氨酸和磷组氨酸。
• Methods for detecting anionic and non-anionic compositions using carbocyanine dyes
  申请人：Gee Richard Kyle

  公开号：US20050244976A1

  公开（公告）日：2005-11-03

  The present invention relates to methods of detecting anionic proteins in a sample with fluorescent carbocyanine dye compounds. The invention also describes methods of simultaneously detecting anionic and non-anionic proteins in a sample with discrete fluorescent signals produced by carbocyanine dye compounds. The invention is of use in a variety of fields including immunology, diagnostics, molecular biology and fluorescence based assays.

  本发明涉及用荧光羰花青染料化合物检测样品中阴离子蛋白质的方法。本发明还描述了用羰花青染料化合物产生的离散荧光信号同时检测样品中阴离子和非阴离子蛋白质的方法。本发明可用于各种领域，包括免疫学、诊断学、分子生物学和基于荧光的检测。