辅酶A,S-[(R)-氢甲基丙二酸酯] | 73173-92-9

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 中文名称: 辅酶A,S-[(R)-氢甲基丙二酸酯]
• 中文别名: 二十二基苯磺化钠
• 英文名称: methylmalonyl-coenzyme A
• 英文别名: (R)-methylmalonyl-CoA；(2R)-3-[2-[3-[[(2R)-4-[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl]oxy-2-hydroxy-3,3-dimethylbutanoyl]amino]propanoylamino]ethylsulfanyl]-2-methyl-3-oxopropanoic acid
• CAS: 73173-92-9
• 化学式: C₂₅H₄₀N₇O₁₉P₃S
• 分子量: 867.615
• InChiKey: MZFOKIKEPGUZEN-AGCMQPJKSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -5.4
• 重原子数：
  55
• 可旋转键数：
  22
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.64
• 拓扑面积：
  426
• 氢给体数：
  10
• 氢受体数：
  24

文献信息

• MICROORGANISMS FOR PRODUCING METHACRYLIC ACID AND METHACRYLATE ESTERS AND METHODS RELATED THERETO
  申请人：BURK Mark J.

  公开号：US20130065279A1

  公开（公告）日：2013-03-14

  The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.

  本发明提供了一种非自然存在的微生物，其具有甲基丙烯酸、甲基丙烯酸酯、3-羟基异丁酸和/或2-羟基异丁酸途径。该微生物含有至少一个外源性核酸，编码甲基丙烯酸途径中的一种酶。本发明还提供了一种生产甲基丙烯酸、甲基丙烯酸酯、3-羟基异丁酸和/或2-羟基异丁酸的方法。该方法可以包括培养产生甲基丙烯酸、甲基丙烯酸酯、3-羟基异丁酸和/或2-羟基异丁酸的微生物，在条件和足够的时间下，微生物表达至少一个外源性核酸编码的甲基丙烯酸途径酶，以产生甲基丙烯酸、甲基丙烯酸酯、3-羟基异丁酸和/或2-羟基异丁酸。
• ENGINEERED MICROORGANISMS AND INTEGRATED PROCESS FOR PRODUCING N-PROPANOL, PROPYLENE AND POLYPROPYLENE
  申请人：Pereira Gonçalo Amarante Guimarães

  公开号：US20130095542A1

  公开（公告）日：2013-04-18

  The invention provides fermentative methods for producing n-propanol. The methods of the invention involve providing a suitable carbon source, a microorganism expressing the dicarboxylic acid pathway, reducing equivalents, and at least one gene coding for an enzyme that catalyzes the conversion of propionate/propionyl-CoA into n-propanol. The methods further involve contacting the carbon source and reducing equivalents with the microorganism under conditions favorable for the production of n-propanol. Also provided are methods for producing propylene and polypropylene from the n-propanol and microorganisms suitable for use in the methods of the invention.

  本发明提供了用于生产正丙醇的发酵方法。该发明的方法涉及提供适当的碳源、表达二羧酸途径的微生物、还原当量和至少一个编码酶的基因，该酶催化丙酸盐/丙酰辅酶A转化为正丙醇。该方法还涉及在有利于生产正丙醇的条件下将碳源和还原当量与微生物接触。本发明还提供了从正丙醇制备丙烯和聚丙烯的方法以及适用于本发明方法的微生物。
• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• METHOD FOR PREPARING POLYLACTATE AND COPOLYMER THEREOF USING A MUTANT MICROORGANISM WITH ENHANCED POLYLACTATE, AND THE COPOLYMER PRODUCING CAPABILITY THEREOF
  申请人：LG Chem, Ltd.

  公开号：EP2314638A2

  公开（公告）日：2011-04-27

  Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.

  本发明提供了一种利用突变微生物制备聚乳酸（PLA）或其共聚物的方法，在这种突变微生物中，参与辅酶A（CoA）供体和乳酸盐产生途径的基因受到遗传操作，以提高CoA供体和乳酸盐的产量。在微生物代谢途径中，CoA 供体和乳酸盐的数量同时增加，从而能够有效地生物合成聚乳酸和具有高乳酸盐含量的羟基烷酸-乳酸盐共聚物，这在工业上是有用的。
• Microorganisms and methods for the co-production of isopropanol with primary alcohols, diols and acids
  申请人：Genomatica, Inc.

  公开号：EP2933338A2

  公开（公告）日：2015-10-21

  The invention provides a non-naturally occurring microbial organism having n-propanol and isopropanol pathways, 1,4-butanediol (14-BDO) and isopropanol pathways, 1,3-butanediol (13- BDO) and isopropanol pathways or methylacrylic acid (MAA) and isopropanolpathways. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in each of the respective n-propanol, 14-BDO, 13-BDO or MAA and isopropanol pathways. The invention additionally provides a method for co-producing n-propanol and isopropanol, 14-BDO and isopropanol, 13-BDO and isopropanol or MAA and isopropanol. The method can include culturing an n-propanol and an isopropanol co-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an n-propanol, an isopropanol, a 14-BDO, a 13-BDO and/or a MAA pathway enzyme in a sufficient amount to produce each of the respective products, under conditions and for a sufficient period of time to produce each of the respective products.

  本发明提供了一种非天然存在的微生物有机体，它具有正丙醇和异丙醇途径、1,4-丁二醇（14-BDO）和异丙醇途径、1,3-丁二醇（13-BDO）和异丙醇途径或甲基丙烯酸（MAA）和异丙醇途径。微生物含有至少一种编码正丙醇、14-BDO、13-BDO 或 MAA 和异丙醇途径中每一种酶的外源核酸。本发明还提供了一种联合生产正丙醇和异丙醇、14-BDO 和异丙醇、13-BDO 和异丙醇或 MAA 和异丙醇的方法。该方法可包括培养正丙醇和异丙醇共产微生物有机体，其中该微生物有机体表达至少一种编码正丙醇、异丙醇、14-BDO、13-BDO 和/或 MAA 途径酶的外源核酸，该核酸的数量足以产生各自的产物，其条件和时间足以产生各自的产物。