Described is a method for the production isoamyl alcohol (3-methylbutan-1-ol) comprising the enzymatic conversion of 3-methylbutyryl-CoA (isovaleryl-CoA) into isoamyl alcohol comprising: (a) two enzymatic steps comprising (i) first the enzymatic conversion of 3-methylbutyryl-CoA into 3-methylbutyraldehyde (3-methylbutanal or isovaleraldehyde); and (ii) then enzymatically converting the thus obtained 3-methylbutyraldehyde into said isoamyl alcohol; or (b) a single enzymatic reaction in which 3-methylbutyryl-CoA is directly converted into isoamyl alcohol by making use of an alcohol-forming short chain acyl-CoA dehydrogenase/fatty acyl-CoA reductase or an alcohol-forming fatty acyl-CoA reductase (long-chain acyl-CoA:NADPH reductase) (EC 1.2.1.84). Further, described is the above method wherein the 3-methylbutyryl-CoA can be provided by the enzymatic conversion of 3-methylcrotonyl-CoA into said 3-methylbutyryl-CoA. It is also described that the thus obtained isoamyl alcohol can be further enzymatically converted into 3-methylbutyl acetate (isoamyl acetate) as described herein. Described are also recombinant organisms or microorganisms which are capable of performing the above enzymatic conversions. Furthermore, described are uses of enzymes and enzyme combinations which allow the above enzymatic conversions.
所描述的是一种生产
异戊醇(3-甲基丁-1-醇)的方法,包括将 3-甲基丁酰-CoA(异戊酰-CoA)酶法转化为
异戊醇,该方法包括:(a) 两个酶解步骤,包括:(i) 首先将 3-甲基丁酰-CoA 酶解转化为
3-甲基丁醛(
3-甲基丁醛或
异戊醛);(ii) 然后将由此获得的
3-甲基丁醛酶解转化为所述
异戊醇;或 (b) 单个酶促反应,利用成醇短链酰基-CoA 脱氢酶/脂肪酰基-CoA 还原酶或成醇脂肪酰基-CoA 还原酶(长链酰基-CoA:
NADPH 还原酶)(
EC 1.2.1.84).此外,还描述了上述方法,其中 3-甲基丁酰基-CoA 可以通过酶法将 3-甲基
巴豆酰基-CoA 转化为所述 3-甲基丁酰基-CoA。还描述了由此获得的
异戊醇可进一步酶促转化为本文所述的 3-甲基
丁基乙酸酯(
乙酸异戊酯)。还描述了能够进行上述酶转化的
重组生物或微
生物。此外,还描述了可进行上述酶转化的酶和酶组合的用途。