4-Carboxy-2-hydroxymuconsaeure | 39118-30-4

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 4-Carboxy-2-hydroxymuconsaeure
• 英文别名: 4-Carboxy-2-hydroxy-cis,cis-muconic acid；(1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylic acid
• CAS: 39118-30-4
• 化学式: C₇H₆O₇
• 分子量: 202.12
• InChiKey: QWLUKZXOQAQUFQ-DXLKSGPOSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.6
• 重原子数：
  14
• 可旋转键数：
  4
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  132
• 氢给体数：
  4
• 氢受体数：
  7

文献信息

• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Conversion of biomass to useful intermediates
  申请人：Alliance for Sustainable Energy, LLC

  公开号：US10253338B2

  公开（公告）日：2019-04-09

  An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.

  本公开的一个方面是一种微生物细胞，它包括导致表达内源二氧 化酶缺陷形式的遗传修饰，以及编码外源二氧 化酶的基因和启动子序列，其中内源二氧 化酶包括 PcaH 和 PcaG、外源二氧化酶包括 LigA 和 LigB，微生物细胞能够利用纤维素分解分子或木质素分解分子中的至少一种进行生长，微生物细胞能够产生 2-羟基-2H-吡喃-4,6-二羧酸。
• Conversion of S-lignin compounds to useful intermediates
  申请人：Alliance for Sustainable Energy, LLC

  公开号：US11136601B2

  公开（公告）日：2021-10-05

  The present disclosure relates to a genetically modified microbial cell that includes a genetic modification resulting in the expression of a vanillate demethylase, where the microbial cell is capable of metabolizing at least one S-lignin decomposition molecule including at least one of syringate and/or 3-O-methyl gallate, and the genetically modified microbial cell is capable of producing gallate. In some embodiments of the present disclosure, the vanillate demethylase may include VanAB.

  本公开涉及一种转基因微生物细胞，它包括导致香草酸脱甲基酶表达的基因修饰，其中微生物细胞能够代谢至少一种S-木质素分解分子，包括丁香酸盐和/或3-O-甲基没食子酸盐中的至少一种，并且转基因微生物细胞能够产生没食子酸盐。在本公开的一些实施方案中，香草酸脱甲基酶可包括 VanAB。
• Engineered microorganisms for the production of intermediates and final products
  申请人：Alliance for Sustainable Energy, LLC

  公开号：US11518975B2

  公开（公告）日：2022-12-06

  The present disclosure relates to a non-naturally occurring microorganism that includes an endogenous genetic deletion that eliminates the expression of at least a pyruvate kinase, where the genetically modified prokaryotic microorganism is capable of producing 3-deoxy-D-arabino-heptulosonate-7-phosphate.

  本公开涉及一种非天然存在的微生物，它包括一种内源基因缺失，至少消除了丙酮酸激酶的表达，其中转基因原核微生物能够生产 3-脱氧-D-阿拉伯糖庚二酸-7-磷酸酯。
• CONVERSION OF BIOMASS TO USEFUL INTERMEDIATES
  申请人：Alliance for Sustainable Energy, LLC

  公开号：US20170275655A1

  公开（公告）日：2017-09-28

  An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.