A deparaffinizing composition and method are provided for removing paraffin wax from biological samples. The deparaffinizing composition may include: a paraffin solvent that may be a first apolar paraffin solvent; a second apolar solvent that may be at least partially miscible with water; a first polar solvent that may be miscible with both water and apolar solvents; a second polar solvent that may be water; and a detergent. The deparaffinizing method may include the steps of: incubating the sample for a time period in a deparaffinizing composition; and rinsing the sample with an aqueous buffer. Preferably, the step of incubating the sample for a time period in the deparaffinizing composition may include heating the sample and the composition to between approximately 35 to 65 degrees Celsius.
提供了一种去石蜡组合物和方法,用于去除
生物样本中的石蜡。去石蜡组合物可以包括:石蜡溶剂,可以是第一极性石蜡溶剂;第二极性溶剂,可以至少部分与
水混溶;第一极性溶剂,可以与
水和极性溶剂混溶;第二极性溶剂,可以是
水;以及洗涤剂。去石墨化方法可包括以下步骤:将样品在去石墨化组合物中培养一段时间;用
水性缓冲液冲洗样品。优选地,将样品在去石蜡组合物中培养一段时间的步骤可包括将样品和组合物加热至大约 35 至 65 摄氏度。