亮氨酰-丝氨酰-脯氨酰-苯丙氨酰-脯氨酰-苯丙氨酰-天冬氨酰-亮氨酸 | 142606-55-1

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: 亮氨酰-丝氨酰-脯氨酰-苯丙氨酰-脯氨酰-苯丙氨酰-天冬氨酰-亮氨酸
• 中文别名: 1-[(2-硝基苯基)磺酰]-1H-吲哚
• 英文名称: Leucyl-seryl-prolyl-phenylalanyl-prolyl-phenylalanyl-aspartyl-leucine
• 英文别名: (2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-2-amino-4-methylpentanoyl]amino]-3-hydroxypropanoyl]pyrrolidine-2-carbonyl]amino]-3-phenylpropanoyl]pyrrolidine-2-carbonyl]amino]-3-phenylpropanoyl]amino]-3-carboxypropanoyl]amino]-4-methylpentanoic acid
• CAS: 142606-55-1
• 化学式: C₄₇H₆₆N₈O₁₂
• 分子量: 935.1
• InChiKey: RLTLDVPHEYUGFZ-QSVFAHTRSA-N

物化性质

• 沸点：
  1307.2±65.0 °C(Predicted)
• 密度：
  1.298±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -0.3
• 重原子数：
  67
• 可旋转键数：
  24
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.55
• 拓扑面积：
  307
• 氢给体数：
  9
• 氢受体数：
  13

制备方法与用途

生物活性

p2Ca是8聚体肽，是天然加工并呈递给Ld同种异体T细胞克隆2C的配体。

体外研究

p2Ca and QL9 peptides assume distinct conformations when bind to Ld and, furthermore, demonstrate that there is flexibility in peptide binding within the MHC class I cleft. Ld antigenic peptide p2Ca (LSPFPFDL) is 8-mer that lack the proline at position 2 and thus use alternative amino-terminal anchors. The p2Ca octamer is identified as the ligand that is naturally processed and presented to the Ld-alloreactive T cell clone, 2C. p2Ca, is immunodominant in allorecognition of the murine MHC class I molecule H-2Ld. The majority of Ld-alloreactive T-cell clones are specific for Ld-p2Ca and this immunodominance is not due to peptide cross-reactivity. p2Ca is a ubiquitously expressed self-peptide. p2Ca is derived from the mouse mitochondrial enzyme α-ketoglutarate dehydrogenase. p2Ca is present in every tissue of BALB/c mice that has been examined, including the spleen and thymus. It is also expressed by mouse tumor cell lines such as the mastocytoma P815. CTL derived in vitro recognize specifically the p2Ca/L d complex and use Vβ8 regions predominantly. The cultured cells lyse target cells with lower levels of p2Ca than the levels used for induction. This result suggests that it may be possible to use peptides at high concentrations to elicit CTL that react with endogenous levels of a peptide/class I complex.

体内研究

BALB/c mice, coinjected with a syngeneic BALB/c myeloma and exogenous p2Ca, are able to reject the tumor. The p2Ca/L d system may thus provide a model for evaluating the parameters for effective immunotherapy with tumor-associated peptides.