trimethylsilyl 11-hydroxy-5,8,12,14-eicosatetraenoic acid methyl ester | 345233-50-3

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 英文名称: trimethylsilyl 11-hydroxy-5,8,12,14-eicosatetraenoic acid methyl ester
• CAS: 345233-50-3
• 化学式: C₂₄H₄₂O₃Si
• 分子量: 406.681
• InChiKey: WTLMFWYCUMSGOL-UHFFFAOYSA-N

反应信息

• 作为产物：
  描述：

  Methyl 11-hydroxyicosa-5,8,12,14-tetraenoate 、 六甲基二硅氮烷 反应 0.5h， 生成 trimethylsilyl 11-hydroxy-5,8,12,14-eicosatetraenoic acid methyl ester
  参考文献：
  名称：

  海藻Ulva的海洋藻类11-脂氧合酶的表征和固定化

  摘要：

  AbstractThe lipoxygenase (LOX) of the marine green alga Ulva fasciata was purified and immobilized in order to improve the stability and reusability. The algal LOX was partially purified by fractionation with 35–55% saturation of ammonium sulfate and MacroPrep high Q anion exchange chromatography. The LOX was purified ten times using linoleic acid (C18:2) or arachidonic acid (C20:4) as substrate, the Michaelis constant (Km) of LOX was 117.6, 31.3 μM, and maximum velocity (Vmax) was 12.8, 23.3 μmol hydroperoxy fatty acid/min‐mg protein, respectively. The algal LOX showed the highest activity towards C18:4 followed by C20:4, C18:2 and methyl ester of C18:4. LOX activity increased up to 10.5 times with increased concentration of Triton X‐100 in the extraction medium reaching an optimum at 0.05%. Calcium chloride, glutathione and phenylmethylsulphonyl fluoride were found effective protectants to LOX during purification. Hydroperoxyeicosatetraenoic acid (HpETE) formed from arachidonic acid catalyzed by this purified algal LOX was reduced and identified as 11‐hydroxy‐5,8,12,14‐eicosatetraenoic acid (11‐HETE) by NP‐HPLC and GC–MS. This algal 11‐LOX was immobilized in alginate beads. The stability was sevenfold greater than that of the unbound lipoxygenase at 4 °C in 0.05 M Tris–HCl buffer (pH 7.5). This is the first report on immobilization of a marine algal lipoxygenase with a view to its potential role in seafood flavor formation.

  DOI：

  10.1007/s11746-008-1262-6