3-Chloro-2,4-hexadienedioic acid | 20665-95-6

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 英文名称: 3-Chloro-2,4-hexadienedioic acid
• 英文别名: (2Z,4E)-3-chlorohexa-2,4-dienedioic acid
• CAS: 20665-95-6
• 化学式: C₆H₅ClO₄
• 分子量: 176.55
• InChiKey: ICMVYBXQDUXEEE-XLFBNKDWSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.6
• 重原子数：
  11
• 可旋转键数：
  3
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  74.6
• 氢给体数：
  2
• 氢受体数：
  4

文献信息

• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Optimization of crossover points for directed evolution
  申请人：Maxygen, Inc.

  公开号：US20030198988A1

  公开（公告）日：2003-10-23

  Methods and devices for more efficiently engineering diversity into recombinant polypeptides and/or nucleic acids are provided herein. For example, a variety of methods of selecting and/or assessing potential crossover sites in an amino acid sequence or a nucleotide sequence are provided, as well as the resulting chimeric product sequences. These methods include, e.g., consideration of structural, functional and/or statistical data in the selection and assessment of sequences and crossover sites for use in recombination.

  本文提供了更有效地将多样性工程转化为重组多肽和/或核酸的方法和装置。例如，提供了多种选择和/或评估氨基酸序列或核苷酸序列中潜在交叉位点的方法，以及由此产生的嵌合产物序列。这些方法包括，例如，在选择和评估用于重组的序列和交叉位点时考虑结构、功能和/或统计数据。
• OPTIMIZATION OF CROSSOVER POINTS FOR DIRECTED EVOLUTION
  申请人：Maxygen, Inc.

  公开号：EP1488335A2

  公开（公告）日：2004-12-22
• EP1488335A4
  申请人：——

  公开号：EP1488335A4

  公开（公告）日：2006-11-15
• US7620500B2
  申请人：——

  公开号：US7620500B2

  公开（公告）日：2009-11-17