N-ethyl-N-n-butyl-ammonium acetate

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: N-ethyl-N-n-butyl-ammonium acetate
• 英文别名: butylethylammonium acetate；n-ethylbutylamine acetate；Butyl(ethyl)azanium;acetate；butyl(ethyl)azanium;acetate
• 化学式: C₂H₄O₂*C₆H₁₅N
• 分子量: 161.244
• InChiKey: KVMHXDIXCIWTCI-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.49
• 重原子数：
  11
• 可旋转键数：
  4
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.88
• 拓扑面积：
  49.3
• 氢给体数：
  2
• 氢受体数：
  3

反应信息

• 作为反应物：
  描述：

  2-methyl-4-phenoxy-8-(2,4,6-trimethylphenyl)-quinoline 、 N-ethyl-N-n-butyl-ammonium acetate 反应 6.0h， 以46%的产率得到2-methyl-4-(N-ethyl-N-n-butyl)amino-8-(2,4,6-trimethylphenyl)-quinoline
  参考文献：
  名称：

  Quinoline and quinazoline derivatives having corticotropin releasing factor (CRF) antagonist activity

  摘要：

  本发明涉及新化合物，涉及制备这些新化合物的方法，涉及含有这些化合物之一或多个作为活性成分的药物组合物，以及使用这些化合物治疗与压力相关的疾病的方法。

  公开号：

  US06350750B1

文献信息

• Compositions and methods for enhancing hybridization specificity
  申请人：Rapigene, Inc.

  公开号：EP0952228A2

  公开（公告）日：1999-10-27

  Compositions and methods are provided for increasing the specificity of a probe nucleic acid for a target nucleic acid in a hybridization solution. An abasic residue, deoxyNebularine residue, or a hybotrope is used to increase specificity. A method is provided for identifying useful hybotropes, including salts, water miscible organic solvents, aprotic solvents and organic solvents, on the basis of enthalpy considerations. Hybotropic hybridization and modified oligonucleotides may be used in amplification reactions, such as PCR, sequence analysis methods, and genomic screening methods.

  本文提供了提高杂交溶液中探针核酸对目标核酸特异性的组合物和方法。使用消旋残基、脱氧星云残基或同源物来提高特异性。本研究提供了一种方法，可根据焓的考虑来确定有用的同素异形体，包括盐类、水溶性有机溶剂、烷基溶剂和有机溶剂。杂交和修饰寡核苷酸可用于扩增反应，如 PCR、序列分析方法和基因组筛选方法。
• Polynucleotide separations on polymeric separation
  申请人：——

  公开号：US20010030156A1

  公开（公告）日：2001-10-18

  Non-polar polymeric separation media, such as beads or monoliths, are suitable for chromatographic separation of mixtures of polynucleotides when the surfaces of the media are unsubstituted or substituted with a hydrocarbon group having from one to 1,000,000 carbons and when the surfaces are substantially free from mutivalent cation contamination. The polymeric media provide efficient separation of polynucleotides using Matched Ion Polynucleotide Chromatography. Methods for maintaining and storing the polymeric media include treatment with multivalent cation binding agents.

  非极性聚合物分离介质（如珠子或单片）适用于多核苷酸混合物的色谱分离，前提是介质表面未被取代或被具有 1 至 1,000,000 个碳原子的烃基取代，且表面基本不受二价阳离子污染。聚合介质可使用匹配离子多核苷酸色谱法有效分离多核苷酸。维护和储存聚合介质的方法包括使用多价阳离子结合剂进行处理。
• Method and system for RNA analysis by matched ion polynucleotide chromatography
  申请人：——

  公开号：US20010042714A1

  公开（公告）日：2001-11-22

  A Matched Ion Polynucleotide Chromatography method and system for size-based segregation of a mixture of RNA molecules. The method includes applying the mixture to a polymeric separation medium having non-polar surfaces and eluting the RNA molecules with a mobile phase which includes counterion reagent and an organic component. The preferred surfaces are characterized by being substantially free from multivalent cations which are free to interfere with RNA segregation. The elution is preferably performed at a temperature sufficient to denature the RNA. The method can be used in segregating RNA molecules having lengths in the range of about 100 to 20,000 nucleotides. Improved segregation is obtained using a chromatography column having an ID greater than about 5 mm. Examples of separation media include beads and monolithic columns.

  一种匹配离子多核苷酸色谱法和系统，用于根据大小分离 RNA 分子混合物。该方法包括将混合物应用于具有非极性表面的聚合物分离介质，并用包括反离子试剂和有机成分的流动相洗脱 RNA 分子。优选表面的特点是基本上不含干扰 RNA 分离的多价阳离子。洗脱最好在足以使 RNA 变性的温度下进行。该方法可用于分离长度在 100 至 20,000 个核苷酸范围内的 RNA 分子。使用内径大于 5 毫米的色谱柱可提高分离效果。分离介质的例子包括珠子和整体柱。
• Polynucleotide separations on polymeric separation media
  申请人：——

  公开号：US20010032817A1

  公开（公告）日：2001-10-25

  Non-polar polymeric separation media, such as beads or monoliths, are suitable for chromatographic separation of mixtures of polynucleotides when the surfaces of the media are unsubstituted or substituted with a hydrocarbon group having from one to 1,000,000 carbons and when the surfaces are substantially free from mutivalent cation contamination. The polymeric media provide efficient separation of polynucleotides using Matched Ion Polynucleolide Chromatography. Methods for maintaining and storing the polymeric media include treatment with multivalent cation binding agents.

  非极性聚合物分离介质（如珠子或单片）适用于多核苷酸混合物的色谱分离，前提是介质表面未被取代或被具有 1 至 1,000,000 个碳原子的烃基取代，且表面基本不受二价阳离子污染。聚合介质可利用匹配离子多核苷酸色谱法有效分离多核苷酸。维护和储存聚合介质的方法包括使用多价阳离子结合剂进行处理。
• Chromatographic method for RNA stabilization
  申请人：——

  公开号：US20010051715A1

  公开（公告）日：2001-12-13

  The instant invention provides a method for stabilizing an RNA molecule against degradation comprising applying a solution to a separation medium having a non-polar separation surface in the presence of a counterion agent, wherein the solution comprises the RNA molecule and an agent capable of catalyzing the degradation of RNA; eluting the RNA molecule from the separation medium by passing through the separation medium a mobile phase containing a concentration of organic solvent sufficient to elute the RNA molecule from the separation medium, where the elution is conducted under conditions that result in a substantial separation of the RNA molecule from the agent capable of catalyzing the degradation of RNA; and collecting an eluant fraction containing the RNA molecule that is substantially free of the agent capable of catalyzing the degradation of RNA. In a preferred embodiment the method is performed under conditions that are substantially free of multivalent cations.

  本发明提供了一种稳定 RNA 分子防止降解的方法，包括在存在反离子剂的情况下，将溶液施用于具有非极性分离表面的分离介质，其中溶液包括 RNA 分子和能够催化 RNA 降解的物质；将含有足以将 RNA 分子从分离介质中洗脱出来的有机溶剂浓度的流动相通过分离介质，从而将 RNA 分子从分离介质中洗脱出来，其中洗脱是在导致 RNA 分子与能够催化 RNA 降解的药剂充分分离的条件下进行的；收集含有 RNA 分子的洗脱液部分，该部分基本上不含能够催化 RNA 降解的药剂。在一个优选的实施方案中，该方法是在基本上不含多价阳离子的条件下进行的。