3-Formyltyrosine

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 3-Formyltyrosine
• 英文别名: 2-amino-3-(3-formyl-4-hydroxyphenyl)propanoic acid
• 化学式: C₁₀H₁₁NO₄
• 分子量: 209.2
• InChiKey: MYVUDDGZMPQJEZ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -2.4
• 重原子数：
  15
• 可旋转键数：
  4
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.2
• 拓扑面积：
  101
• 氢给体数：
  3
• 氢受体数：
  5

文献信息

• [EN] UNUSUAL SUBSTRATES OF TUBULIN TYROSINE LIGASE<br/>[FR] SUBSTRATS INHABITUELS DE TUBULINE-TYROSINE LIGASE
  申请人：FORSCHUNGSVERBUND BERLIN EV

  公开号：WO2017186855A1

  公开（公告）日：2017-11-02

  The present invention provides means and methods for functionalizing a polypeptide of interest at its C-terminus with an amino acid derivative.

  本发明提供了一种在其C-末端用氨基酸衍生物对感兴趣的多肽进行功能化的手段和方法。
• CD30 TARGETING ANTIBODY DRUG CONJUGATES AND USES THEREOF
  申请人：Ludwig-Maximilians-Universitaet Muenchen

  公开号：US20220193251A1

  公开（公告）日：2022-06-23

  The present invention relates to an antibody-drug conjugate (ADC) comprising: (a) Brentuximab, wherein Brentuximab comprises at the C-terminus of the light chains, the heavy chains or all of the heavy and light chains of the Brentuximab a recognition sequence for tubulin tyrosine ligase and a non-natural amino acid; and (b) at least one drug moiety; wherein a drug moiety is coupled to each of the non-natural amino acids via a linker. The present invention further relates to methods of producing same, pharmaceutical compositions comprising same as well as uses thereof.

  本发明涉及一种抗体药物偶联物（ADC），包括：（a）Brentuximab，其中Brentuximab在轻链、重链或所有重链和轻链的C-末端具有识别微管蛋白酪氨酸连接酶的识别序列和非天然氨基酸；以及（b）至少一种药物基团；其中每个药物基团通过连接剂连接到每个非天然氨基酸上。本发明还涉及制备该ADC的方法、包含该ADC的制药组合物以及其用途。
• Site-specific orthogonal labeling of the carboxy terminus of α-tubulin in live cells
  申请人：The Research Foundation for The State University of New York

  公开号：US10071990B2

  公开（公告）日：2018-09-11

  A technique is provided to visualize microtubules in live cells that does not require genetic manipulation or microinjection. Moreover, this method also avoids perturbation of the endogenous microtubule network that occurs with taxol treatment. This technique exploits tyrosination and detyrosination of tubulin, a posttranslational modification cycle specific to the C-terminus of α-tubulin. Specifically, cells are grown in medium supplemented with a tyrosine derivative possessing a reactive functional group. The cellular enzyme tubulin tyrosine ligase attaches the unnatural amino acid to a single site on tubulin. Addition of fresh medium containing a suitably derivatized fluorophore then yields fluorescent tubulin, which incorporate into cellular microtubules. Importantly, the tubulin labeling approach demonstrated here does not detrimentally affect microtubule network or cell morphology. Thus we present a simple, robust labeling technique that allows microscopic analysis of microtubules in live cells.

  本研究提供了一种无需基因操作或显微注射即可观察活细胞中微管的技术。此外，这种方法还避免了紫杉醇处理对内源性微管网络的干扰。这种技术利用了微管蛋白的酪氨酸化和脱酪氨酸化，这是一种针对α-微管蛋白C末端的翻译后修饰周期。具体来说，细胞在添加了具有活性官能团的酪氨酸衍生物的培养基中生长。细胞中的微管蛋白酪氨酸连接酶将非天然氨基酸连接到微管蛋白上的一个位点上。然后加入含有适当衍生荧光团的新鲜培养基，产生荧光小管蛋白，并与细胞微管结合。重要的是，这里展示的微管蛋白标记方法不会对微管网络或细胞形态产生不利影响。因此，我们提出了一种简单、稳健的标记技术，可以对活细胞中的微管进行显微分析。
• Means and methods for site-specific functionalization of polypeptides
  申请人：LUDWIG-MAXIMILIANS-UNIVERSITÄT MÜNCHEN

  公开号：US10208084B2

  公开（公告）日：2019-02-19

  The present invention provides means and methods for equipping a polypeptide of interest at its C-terminus with a versatile adaptor amino acid that allows the functionalization of the polypeptide of interest.

  本发明提供了在感兴趣的多肽的 C 端配备通用适配氨基酸的方法和手段，这种适配氨基酸可使感兴趣的多肽功能化。
• Processes and host cells for genome, pathway, and biomolecular engineering
  申请人：enEvolv, Inc.

  公开号：US10370654B2

  公开（公告）日：2019-08-06

  The present disclosure provides compositions and methods for genomic engineering.

  本公开提供了基因组工程的组合物和方法。