dTDP-D-glucose

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘧啶核苷酸
• 英文名称: dTDP-D-glucose
• 英文别名: [hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] [(3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] hydrogen phosphate
• 化学式: C₁₆H₂₆N₂O₁₆P₂
• 分子量: 564.334
• InChiKey: YSYKRGRSMLTJNL-KFQCIAAJSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -5.5
• 重原子数：
  36
• 可旋转键数：
  9
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.75
• 拓扑面积：
  271
• 氢给体数：
  8
• 氢受体数：
  16

反应信息

• 作为反应物：
  描述：

  HEPES NaOH 、 尿苷(5')二氢二磷酰(1)-alpha-D-葡萄糖 、 dTDP-D-glucose 以For both enzymes, the Mops-buffer and the TES-buffer gave 60 to 80% residual activity的产率得到蔗糖
  参考文献：
  名称：

  Purified saccharose-synthase, process for its production and its use

  摘要：

  核苷酸糖，特别是UDP、ADP、CDP或TDP蔗糖，可以通过核苷酸二磷酸与二糖或三糖在蔗糖合成酶的催化下反应而得到。通过特殊的纯化方法和敏感的检测，可以确保核苷酸磷酸酶的虚拟缺失（0.1%或更少）。从最好从稻谷中获得的原始提取物的纯化，尤其包括在pH 8下在Sephadex Q柱上应用含50mM KCl的超滤提取物，然后在pH 8下以50至500mM KCl的梯度洗脱出柱。

  公开号：

  US05750389A1
• 作为产物：
  描述：

  胸苷-5'-三磷酸 、 对氟苯甲醇 在 Kitasatospora kifunensis MJM₃₄₁ recombinant RmlA protein 作用下， 生成 dTDP-D-glucose
  参考文献：
  名称：

  Cloning and in vitro characterization of dTDP-6-deoxy-l-talose biosynthetic genes from Kitasatospora kifunensis featuring the dTDP-6-deoxy-l-lyxo-4-hexulose reductase that synthesizes dTDP-6-deoxy-l-talose

  摘要：

  Kitasatospora kifunensis, the talosin producer, was used as a source for the dTDP-6-deoxy-L-talose (dTDP-6dTal) biosynthetic gene cluster, serving as a template for four recombinant proteins of RmlA(Kkf), RmlB(Kkf), RmlC(Kkf), and Tal, which complete the biosynthesis of dTDP-6dTal from dTTP, alpha-D-glucose-1-phosphate, and NAD(P)H. The identity of dTDP-6dTal was validated using H-1 and C-13 NMR spectroscopy. K. kifunensis tal and tll, the known dTDP-6dTal synthase gene of Actinobacillus actinomycetemcomitans origin, have low sequence similarity and are distantly related within the NDP-6-deoxy-4-ketohexose reductase family, providing an example of the genetic diversity within the dTDP-6dTal biosynthetic pathway. (C) 2010 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.carres.2010.07.004

文献信息

• Cloning and in vitro characterization of dTDP-6-deoxy-l-talose biosynthetic genes from Kitasatospora kifunensis featuring the dTDP-6-deoxy-l-lyxo-4-hexulose reductase that synthesizes dTDP-6-deoxy-l-talose
  作者：Suman Karki、Hye-Gyeong Yoo、So-Yeon Kwon、Joo-Won Suh、Hyung-Jin Kwon

  DOI：10.1016/j.carres.2010.07.004

  日期：2010.9

  Kitasatospora kifunensis, the talosin producer, was used as a source for the dTDP-6-deoxy-L-talose (dTDP-6dTal) biosynthetic gene cluster, serving as a template for four recombinant proteins of RmlA(Kkf), RmlB(Kkf), RmlC(Kkf), and Tal, which complete the biosynthesis of dTDP-6dTal from dTTP, alpha-D-glucose-1-phosphate, and NAD(P)H. The identity of dTDP-6dTal was validated using H-1 and C-13 NMR spectroscopy. K. kifunensis tal and tll, the known dTDP-6dTal synthase gene of Actinobacillus actinomycetemcomitans origin, have low sequence similarity and are distantly related within the NDP-6-deoxy-4-ketohexose reductase family, providing an example of the genetic diversity within the dTDP-6dTal biosynthetic pathway. (C) 2010 Elsevier Ltd. All rights reserved.
• Purified saccharose-synthase, process for its production and its use
  申请人：Forschungszentrum Julich GmbH

  公开号：US05750389A1

  公开（公告）日：1998-05-12

  Nucleotide sugars, especially UDP, ADP, CDP or TDP saccharoses can be enzymatically obtained by the reaction of nucleoside diphosphates with di or trisaccharides with a saccharose synthase in which the virtual absence of nucleoside phosphatases (0.1% or less) can be ensured by special purification methods and sensitive detection. The purification of the raw extract, obtained preferably from rice grains, comprises especially the application of the ultra-filtered extract containing 50 mM KCl with a pH 8 on a sepharose Q column and a gradient elution out of the column at a pH 8 with 50 to 500 mM KCl.

  核苷酸糖，特别是UDP、ADP、CDP或TDP蔗糖，可以通过核苷酸二磷酸与二糖或三糖在蔗糖合成酶的催化下反应而得到。通过特殊的纯化方法和敏感的检测，可以确保核苷酸磷酸酶的虚拟缺失（0.1%或更少）。从最好从稻谷中获得的原始提取物的纯化，尤其包括在pH 8下在Sephadex Q柱上应用含50mM KCl的超滤提取物，然后在pH 8下以50至500mM KCl的梯度洗脱出柱。