The invention describes an improved stable reagent composition and method for the determination of hemoglobin (Hb) using whole blood samples and automated hematology analyzers, for example, the TECHNICON H*™ series of instruments as sold by the assignee hereof. The Hb reagent comprises an inorganic cyanide salt, at least one surfactant and a base and has a final pH of about 10.0 to about 11.2, preferably about 10.4 to about 11.2 or less and more preferably about 10.8 to about 11.2 or less. This reagent is intended for use as a universal Hb determination reagent for all of the instruments in the aforementioned series and will provide accurate and precise results without accompanying interference from elevated numbers of white blood cells, especially as found in abnormal blood specimens, and without system-to-system variation in the results obtained.
本发明描述了一种改进的稳定试剂组合物和方法,用于使用全血样品和自动血液分析仪(例如受让方出售的
TECHNICON H*™ 系列仪器)测定血红蛋白 (Hb)。Hb 试剂由无机
氰化盐、至少一种表面活性剂和碱组成,最终 pH 值约为 10.0 至约 11.2,优选约 10.4 至约 11.2 或更低,更优选约 10.8 至约 11.2 或更低。该试剂可用作上述系列所有仪器的通用 Hb 测定试剂,可提供准确、精确的结果,不会受到白细胞数量升高的干扰,尤其是在异常血液标本中发现的白细胞数量升高的干扰,所获得的结果也不会出现系统与系统之间的差异。