Atto 655 | 1258238-14-0

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并恶嗪类
• 英文名称: Atto 655
• 英文别名: Atto 655, BioReagent, suitable for fluorescence, >=85% (HPLC)；[6-(3-carboxypropyl)-20-ethyl-7,7-dimethyl-2-oxa-6,13-diaza-20-azoniapentacyclo[12.8.0.03,12.05,10.016,21]docosa-1(22),3(12),4,10,13,15,20-heptaen-9-yl]methanesulfonate
• CAS: 1258238-14-0
• 化学式: C₂₇H₃₃N₃O₆S
• 分子量: 527.642
• InChiKey: FOYVTVSSAMSORJ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.6
• 重原子数：
  37
• 可旋转键数：
  6
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.52
• 拓扑面积：
  131
• 氢给体数：
  1
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  Fmoc-O-叔丁基-L-谷氨酸 、 Fmoc-Lys(Dde)-OH 、 Fmoc-Pbf-L-精氨酸 、 蝶酸 、 Atto 655 在 N-甲基吗啉 、 1-羟基苯并三唑 、 O-(1H-benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate 、 哌啶 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 2.0h， 生成
  参考文献：
  名称：

  A folate receptor-specific activatable probe for near-infrared fluorescence imaging of ovarian cancer

  摘要：

  合成了一种叶酸受体特异性可激活探针，用于体内近红外荧光成像，具有高的目标-背景比。

  DOI：

  10.1039/c4cc02301c

文献信息

• NUCLEIC ACID PROBE FOR ASSAYING NUCLEIC ACIDS
  申请人：Nippon Steel & Sumikin Eco-Tech Corporation

  公开号：EP2722388A1

  公开（公告）日：2014-04-23

  Objects are to provide nucleic acid probes, which in the detection of target nucleic acids with fluorescent quenching probes, can assay a greater variety of target nucleic acids at the same time while suppressing increases in assay labor and cost, and also a method for assaying target nucleic acids by using the nucleic acid probes. Provided is a nucleic acid probe having a base sequence hybridizable with a target nucleic acid sequence in a target nucleic acid. At least one terminal base is labeled with a fluorescence quenching dye, the base labeled with the fluorescence quenching dye is cytosine, the base sequence is designed such that upon hybridization of the nucleic acid probe with the target nucleic acid sequence, at least one guanine base exists in a range of five bases centering at the base in the target nucleic acid sequence, the base being to be paired with the cytosine base, and the fluorescence quenching dye is a fluorescence quenching dye selected from the group consisting of ATTO465, ATTO655, ATT0680 and ATTO700.

  目的是提供一种核酸探针，它在用荧光淬灭探针检测目标核酸时，可以同时检测更多种类的目标核酸，同时抑制检测人力和成本的增加，还提供一种使用该核酸探针检测目标核酸的方法。 所提供的核酸探针具有可与目标核酸中的目标核酸序列杂交的碱基序列。至少一个末端碱基被荧光淬灭染料标记，被荧光淬灭染料标记的碱基是胞嘧啶，碱基序列被设计成当核酸探针与目标核酸序列杂交时，至少存在一个鸟嘌呤碱基、荧光淬灭染料是从 ATTO465、ATTO655、ATT0680 和 ATTO700 所组成的组中选出的荧光淬灭染料。
• METHOD FOR DETECTING SINGLE BASE SUBSTITUTION USING ION EXCHANGE CHROMATOGRAPHY
  申请人：Sekisui Medical Co., Ltd.

  公开号：EP3690039A1

  公开（公告）日：2020-08-05

  An object of the present invention is to provide a method for accurately and quantitatively discriminating and detecting a wide variety of gene mutations, or particularly, single base substitutions or point mutations. In an ASP for analyzing gene mutations, or particularly, single base substitutions or point mutations, when a non-nucleotide component is added to the 5' end of at least one of the ASP and a primer paired therewith before amplification by PCR and amplification products thereof are separated by ion-exchange chromatography, even the amplification products having the same length can be separated and detected.

  本发明的目的是提供一种方法，用于准确和定量地鉴别和检测各种基因突变，特别是单碱基替换或点突变。在用于分析基因突变，特别是单碱基置换或点突变的 ASP 中，如果在 PCR 扩增前在至少一种 ASP 的 5'端和与之配对的引物中加入非核苷酸成分，并用离子交换色谱法分离其扩增产物，即使是具有相同长度的扩增产物也能被分离和检测出来。
• EP2722388B1
  申请人：——

  公开号：EP2722388B1

  公开（公告）日：2017-08-09
• Fluoroimmunoassay Method
  申请人：USHIO DENKI KABUSHIKI KAISHA

  公开号：US20160349266A1

  公开（公告）日：2016-12-01

  An object is to provide an immunoassay method requiring neither a solid-phase immobilization step nor a washing step, enabling quick and simple quantitative measurement of a target substance in a liquid phase and capable of visualizing an antigen. Such an object is attained by measuring the concentration of a target antigen present in a test substance by sequentially performing a step (a) of bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; a step (b) of measuring the fluorescence intensity of the fluorescent dye; and a step (c) of computationally obtaining the level of the antigen contained in the test substance with reference to a positive correlation between the concentration of the antigen in a liquid phase and the fluorescence intensity of the fluorescent dye.