1-(5-methoxy-2-nitro-4-prop-2-ynyloxyphenyl)ethyl N-succinimidyl carbonate | 1255792-06-3

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 1-(5-methoxy-2-nitro-4-prop-2-ynyloxyphenyl)ethyl N-succinimidyl carbonate
• 英文别名: (2,5-Dioxopyrrolidin-1-yl) 1-(5-methoxy-2-nitro-4-prop-2-ynoxyphenyl)ethyl carbonate；(2,5-dioxopyrrolidin-1-yl) 1-(5-methoxy-2-nitro-4-prop-2-ynoxyphenyl)ethyl carbonate
• CAS: 1255792-06-3
• 化学式: C₁₇H₁₆N₂O₉
• 分子量: 392.322
• InChiKey: MKSWGFYOUJLBSB-UHFFFAOYSA-N

物化性质

• 沸点：
  548.2±60.0 °C(Predicted)
• 密度：
  1.45±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  1.4
• 重原子数：
  28
• 可旋转键数：
  8
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.35
• 拓扑面积：
  137
• 氢给体数：
  0
• 氢受体数：
  9

反应信息

• 作为反应物：
  描述：

  1-(5-methoxy-2-nitro-4-prop-2-ynyloxyphenyl)ethyl N-succinimidyl carbonate 、 雷帕霉素 在 4-二甲氨基吡啶 作用下， 以 二氯甲烷 为溶剂， 反应 20.0h， 以45%的产率得到
  参考文献：
  名称：

  Light-cleavable rapamycin dimer as an optical trigger for protein dimerization

  摘要：

  使用可光解的拉帕霉素二聚体，可以光学控制FKBP12和FRB的蛋白质异二聚化。

  DOI：

  10.1039/c4cc09442e
• 作为产物：
  描述：

  N,N'-二琥珀酰亚胺基碳酸酯 、 3-溴丙炔 、 1-(4-羟基-5-甲氧基-2-硝基苯基)乙酮 在 potassium carbonate 、 sodium tetrahydroborate 、 三乙胺 作用下， 以 乙腈 、 四氢呋喃 、 甲醇 为溶剂， 反应 11.0h， 生成 1-(5-methoxy-2-nitro-4-prop-2-ynyloxyphenyl)ethyl N-succinimidyl carbonate
  参考文献：
  名称：

  一种光敏性聚乙二醇基抗菌水凝胶敷料及其 制备方法

  摘要：

  本发明涉及一种光敏性聚乙二醇基抗菌水凝胶敷料及其制备方法，该敷料是在聚乙二醇水凝胶基体上共价接枝有抗生素。其制备方法包括：将小分子抗生素通过紫外光可断裂分子修饰到末端带双键的聚乙二醇大分子链，得到紫外光可断裂的聚乙二醇基大分子，与未修饰的含末端双键的聚乙二醇分子共混作为单体，在交联剂、引发剂作用下通过热引发双键交联制备得到具有光敏性抗菌性能的水凝胶敷料。本发明的原料具有良好的生物形容性，得到的水凝胶敷料具有一定的力学性能和吸水溶胀性能，且在365nm波长的紫外光照射下能够实现抗生素的有效控制释放，具备良好的紫外光响应性抗菌性能，适用于创面的保护和感染治疗。

  公开号：

  CN108976758B

文献信息

• 3D-exoquant method for the analysis of surface molecules and quantification of tissue-specific exosomes in biological fluids
  申请人：The Regents of the University of California

  公开号：US11243215B2

  公开（公告）日：2022-02-08

  In various embodiments methods are provided for identifying and/or quantifying one or more antigens of interest (biomarkers) on the surface of cell- or tissue-specific exosomes. In an illustrative embodiments the methods comprise: i) incubating a population of exosomes with one or more tissue-specific antibodies that bind an antigen specific to a tissue or cell type of interest that produces exosomes, where the tissue specific antibodies are attached to acceptor bead or magnetic beads so the antibodies bind exosomes displaying the antigen; ii) obtaining a purified population of exosomes bound by the tissue specific antibodies with and/or without photocleavable linker based technology; iii) incubating a test subset of the isolated tissue-specific exosomes with acceptor beads attached to test antibodies that bind an antigen of interest thereby binding exosomes that display the antigen of interest and a control subset with negative control acceptor beads; v) incubating the test subset of isolated exosomes and the control subset of exosomes with a donor-bearing antibody that binds an exosome specific antigen; and vi) detecting a signal produced upon illumination of the control subset and/or the test; and vii) detecting the antigen(s) of interest.

  在各种实施例中，提供了用于识别和/或定量细胞或组织特异性外泌体表面上的一个或多个感兴趣的抗原（生物标志物）的方法。在一个说明性实施例中，该方法包括：i）将外泌体群体与一个或多个组织特异性抗体孵育，这些抗体与特定于产生外泌体的感兴趣组织或细胞类型的抗原结合，其中这些组织特异性抗体附着在受体珠或磁珠上，使抗体结合显示抗原的外泌体；ii）获得由组织特异性抗体结合的外泌体的纯化群体，有时使用光解链技术；iii）将分离的组织特异性外泌体的测试子群体与附着有结合感兴趣抗原的测试抗体的受体珠孵育，从而结合显示感兴趣抗原的外泌体，以及一个带有阴性对照受体珠的对照子群体；v）将分离的外泌体的测试子群体和对照子群体与结合外泌体特异性抗原的供体抗体孵育；vi）在照射对照子群体和/或测试子群体时检测产生的信号；以及vii）检测感兴趣的抗原。
• Vitamin B₁₂ Derivatives Suitably Tailored for the Synthesis of Photolabile Conjugates
  作者：Agnieszka Jackowska、Dorota Gryko

  DOI：10.1021/acs.orglett.1c00839

  日期：2021.7.2

  we decided to develop a method for the preparation of vitamin B12 conjugates that release tethered molecules upon exposure to light. Herein, we report vitamin B12 derivatives possessing a photolabile linker suitable for conjugation with amines, azides, and alkynes. The potential applications of such conjugates are broad and include the delivery of drugs, labels, and imaging agents to their place of

  维生素 B 12结合物在生物科学中得到广泛研究。由于光提供时空控制，我们决定开发一种制备维生素 B 12结合物的方法，该结合物在暴露于光时会释放束缚分子。在此，我们报告了维生素 B 12衍生物，其具有适合与胺、叠氮化物和炔烃结合的光不稳定接头。这种偶联物的潜在应用是广泛的，包括将药物、标签和显像剂输送到它们的作用位置和时空释放。
• [EN] LIGHT MEDIATED REGULATION OF PROTEIN DIMERIZATION<br/>[FR] RÉGULATION MÉDIÉE PAR LA LUMIÈRE DE LA DIMÉRISATION DE PROTÉINE
  申请人：UNIV NORTH CAROLINA

  公开号：WO2012024558A3

  公开（公告）日：2012-07-05
• 3D-EXOQUANT METHOD FOR THE ANALYSIS OF SURFACE MOLECULES AND QUANTIFICATION OF TISSUE-SPECIFIC EXOSOMES IN BIOLOGICAL FLUIDS
  申请人：The Regents of the University of California

  公开号：US20190025330A1

  公开（公告）日：2019-01-24

  In various embodiments methods are provided for identifying and/or quantifying one or more antigens of interest (biomarkers) on the surface of cell- or tissue-specific exosomes. In an illustrative embodiments the methods comprise: i) incubating a population of exosomes with one or more tissue-specific antibodies that bind an antigen specific to a tissue or cell type of interest that produces exosomes, where the tissue specific antibodies are attached to acceptor bead or magnetic beads so the antibodies bind exosomes displaying the antigen; ii) obtaining a purified population of exosomes bound by the tissue specific antibodies with and/or without photo-cleavable linker based technology; iii) incubating a test subset of the isolated tissue-specific exosomes with acceptor beads attached to test antibodies that bind an antigen of interest thereby binding exosomes that display the antigen of interest and a control subset with negative control acceptor beads; v) incubating the test subset of isolated exosomes and the control subset of exosomes with a donor-bearing antibody that binds an exosome specific antigen; and vi) detecting a signal produced upon illumination of the control subset and/or the test; and vii) detecting the antigen(s) of interest.
• Light-cleavable rapamycin dimer as an optical trigger for protein dimerization
  作者：Kalyn A. Brown、Yan Zou、David Shirvanyants、Jie Zhang、Subhas Samanta、Pavan K. Mantravadi、Nikolay V. Dokholyan、Alexander Deiters

  DOI：10.1039/c4cc09442e

  日期：——

  Protein heterodimerization of FKBP12 and FRB can be optically controlled with a photocleavable rapamycin dimer.

  使用可光解的拉帕霉素二聚体，可以光学控制FKBP12和FRB的蛋白质异二聚化。