ethyl 2,2-difluoro-5,5-dimethylhexanoate | 96056-60-9

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 英文名称: ethyl 2,2-difluoro-5,5-dimethylhexanoate
• CAS: 96056-60-9
• 化学式: C₁₀H₁₈F₂O₂
• 分子量: 208.249
• InChiKey: UXVVSIDUZPULSQ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.6
• 重原子数：
  14
• 可旋转键数：
  6
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.9
• 拓扑面积：
  26.3
• 氢给体数：
  0
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  ethyl 2-oxo-5,5-dimethylhexanoate 以 二氯甲烷 为溶剂， 以1.0 g(25%)的产率得到ethyl 2,2-difluoro-5,5-dimethylhexanoate
  参考文献：
  名称：

  Fluoroketone enzyme inhibitors

  摘要：

  一种用于酶免疫分析的方法，用于检测液体样品中可能存在的配体，包括利用至少两种酶和一个用于其中一种酶的阻断调节剂进行信号放大。液体中存在的配体与抗配体和标记酶的示踪剂结合。然后分离所得的结合分数，并且示踪剂中的酶去除阻断调节剂上的阻断基团。调节剂激活或抑制第二种酶，该酶催化底物转化为产物。液体中配体的存在或缺失由与产物相关的信号指示，例如颜色变化或颜色变化速率。该发明包括一类新型酶抑制剂和阻断抑制剂，以及用于执行该发明方法的一套有用材料。

  公开号：

  US05344952A1

文献信息

• Signal enhancement in immunoassay by inhibition of enzymatic catalysis
  申请人：Becton Dickinson and Company

  公开号：EP0271731A2

  公开（公告）日：1988-06-22

  A method for enzyme immunoassay for a ligand suspected to be present in a liquid sample includes signal amplification by use of at least two enzymes and a blocked modulator for one of the enzymes. Ligand present in the liquid binds to an antiligand and an enzyme-labeled tracer. The resulting bound fraction is separated and the enzyme in the tracer removes the blocking group from the blocked modulator. The modulator activates or inhibits a second enzyme which catalyzes the conversion of a substrate to a product. The presence or absence of the ligand in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the product. The invention includes a new class of enzyme inhibitors and blocked inhibitors and a kit of materials useful for performing the method of the invention.

  一种对怀疑存在于液体样品中的配体进行酶免疫测定的方法，包括使用至少两种酶和其中一种酶的阻断调节剂进行信号放大。 液体中存在的配体与抗配体和酶标记的示踪剂结合。 由此产生的结合部分被分离，示踪剂中的酶从阻断调节剂中去除阻断基团。 调节剂激活或抑制第二种酶，该酶催化底物向产物的转化。 液体中配体的存在与否通过与产物相关的信号（如颜色变化或颜色变化率）来指示。 本发明包括一类新的酶抑制剂和阻断抑制剂，以及一套用于实施本发明方法的材料。
• Signal enhancement in assay for an enzyme
  申请人：Becton Dickinson and Company

  公开号：EP0354548A2

  公开（公告）日：1990-02-14

  A method for assay for an unknown enzyme suspected to be present in a liquid includes signal amplification by use of a second enzyme and a blocked modulator for the second enzyme. Unknown enzyme in the liquid removes a blocking group from the blocked modulator. The resulting modulator activates or inhibits the second enzyme which catalyzes an indicator reaction in which a substrate is converted to a product. The presence or absence of the unknown enzyme in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the indicator reaction. The concentration of the enzyme in the sample may be determined by the measurement of the signal. The invention includes a kit of materials useful for performing the method of the invention.

  一种检测液体中疑似存在的未知酶的方法，包括使用第二种酶和第二种酶的阻断调制剂进行信号放大。液体中的未知酶会从阻断调制剂中去除阻断基团。由此产生的调制剂激活或抑制第二种酶，第二种酶催化指示剂反应，其中底物转化为产物。液体中未知酶的存在与否可通过与指示剂反应相关的信号（如颜色变化或颜色变化率）来显示。样品中酶的浓度可通过测量信号来确定。本发明包括一套用于实施本发明方法的材料。
• Fluoroketones as blocked enzyme inhibitors for use in immunoassays
  申请人：Becton Dickinson and Company

  公开号：EP0501526A2

  公开（公告）日：1992-09-02

  A method for enzyme immunoassay for a ligand suspected to be present in a liquid sample includes signal amplification by use of at least two enzymes and a blocked modulator for one of the enzymes. Ligand present in the liquid binds to an antiligand and an enzyme-labeled tracer. The resulting bound fraction is separated and the enzyme in the tracer removes the blocking group from the blocked modulator. The modulator activates or inhibits a second enzyme which catalyzes the conversion of a substrate to a product. The presence or absence of the ligand in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the product. The invention includes a new class of enzyme inhibitors and blocked inhibitors and a kit of materials useful for performing the method of the invention.

  一种对液体样品中疑似存在的配体进行酶免疫测定的方法，包括使用至少两种酶和其中一种酶的阻断调节剂进行信号放大。液体中的配体与抗配体和酶标记的示踪剂结合。由此产生的结合部分被分离，示踪剂中的酶从阻断调制剂中去除阻断基团。调制剂激活或抑制第二种酶，该酶催化底物向产物的转化。液体中配体的存在与否可通过与产物相关的信号（如颜色变化或颜色变化率）来显示。本发明包括一类新的酶抑制剂和阻断抑制剂，以及用于实施本发明方法的材料试剂盒。
• US4835099A
  申请人：——

  公开号：US4835099A

  公开（公告）日：1989-05-30
• US4962024A
  申请人：——

  公开号：US4962024A

  公开（公告）日：1990-10-09