6-([3,4-trimethylene]anilino)uracil | 64054-69-9

• 分子结构分类: 有机化合物 - 苯类化合物 - 茚满类
• 英文名称: 6-([3,4-trimethylene]anilino)uracil
• 英文别名: 6-indan-5-ylamino-1H-pyrimidine-2,4-dione；6-(Indan-5-ylamino)-1H-pyrimidine-2,4-dione；6-(2,3-dihydro-1H-inden-5-ylamino)-1H-pyrimidine-2,4-dione
• CAS: 64054-69-9
• 化学式: C₁₃H₁₃N₃O₂
• 分子量: 243.265
• InChiKey: CVUMGFDAIXWWCV-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.6
• 重原子数：
  18
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.23
• 拓扑面积：
  70.2
• 氢给体数：
  3
• 氢受体数：
  3

文献信息

• Method for measuring dna polymerization and applications of the method
  申请人：——

  公开号：US20040157230A1

  公开（公告）日：2004-08-12

  A method for measuring DNA-dependent DNA polymerisation in a biological sample, is described. The method comprises the steps of providing a primer with a single stranded short specific sequence, which is unable to base pair internally, bound to a solid phase; contacting the primer construct with a reaction mixture containing a single stranded deoxynucleotide template with a part of the sequence complementary to the primer and the four deoxynucleoside triphosphates, one of which is modified so that it is specifically recognized by a labeled antibody; adding a biological sample comprising the DNA polymerase, such as retrovirus reverse transcriptase (RT), to the mixture; allowing the polymerase reaction to proceed; incubating the immobilized reaction product with the labeled antibody; detecting the amount of bound labeled antibody; and measuring the amount of incorporated modified deoxynucleoside triphosphate, as a measure of the DNA polymerisation, which may be used drug susceptibility testing. A commercial package is also disclosed.

  描述了一种测量生物样本中依赖 DNA 的 DNA 聚合的方法。该方法包括以下步骤：提供与固相结合的具有单链短特异序列的引物，该引物内部不能进行碱基配对；将引物构建体与含有单链脱氧核苷酸模板的反应混合物接触，该模板具有与引物互补的部分序列和四种脱氧核苷三磷酸，其中一种脱氧核苷三磷酸经过修饰，可被标记的抗体特异性识别；将包含 DNA 聚合酶（如逆转录病毒逆转录酶 (RT)）的生物样本加入混合物中；使聚合酶反应继续进行；将固定化的反应产物与标记抗体孵育；检测结合的标记抗体的量；测量结合的修饰脱氧核苷三磷酸的量，作为 DNA 聚合的量度，可用于药敏试验。此外，还公开了一种商业包装。
• A METHOD FOR MEASURING DNA POLYMERIZATION AND APPLICATIONS OF THE METHOD
  申请人：Cavidi Tech AB

  公开号：EP1395675A1

  公开（公告）日：2004-03-10
• US7488577B2
  申请人：——

  公开号：US7488577B2

  公开（公告）日：2009-02-10
• [EN] A METHOD FOR MEASURING DNA POLYMERIZATION AND APPLICATIONS OF THE METHOD<br/>[FR] PROCEDE POUR MESURER LA POLYMERISATION DE L'ADN ET APPLICATIONS DU PROCEDE
  申请人：CAVIDI TECH AB

  公开号：WO2002103039A1

  公开（公告）日：2002-12-27

  A method for measuring DNA-dependent DNA polymerisation in a biological sample, is described. The method comprises the steps of providing a primer with a single stranded short specific sequence, which is unable to base pair internally, bound to a solid phase; contacting the primer construct with a reaction mixture containing a single stranded deoxynucleotide template with a part of the sequence complementary to the primer and the four deoxynucleoside triphosphates, one of which is modified so that it is specifically recognized by a labeled antibody; adding a biological sample comprising the DNA polymerase, such as retrovirus reverse transcriptase (RT), to the mixture; allowing the polymerase reaction to proceed; incubating the immobilized reaction product with the labeled antibody; detecting the amount of bound labeled antibody; and measuring the amount of incorporated modified deoxynucleoside triphosphate, as a measure of the DNA polymerisation, which may be used drug susceptibility testing. A commercial package is also disclosed.