2-(Hexadecanoylamino)ethyl-trimethylazanium

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 英文名称: 2-(Hexadecanoylamino)ethyl-trimethylazanium
• 化学式: C₂₁H₄₅N₂O
• 分子量: 341.601
• InChiKey: TUVXPFGBAXGSOG-UHFFFAOYSA-O

计算性质

• 辛醇/水分配系数(LogP)：
  6.9
• 重原子数：
  24
• 可旋转键数：
  17
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.95
• 拓扑面积：
  29.1
• 氢给体数：
  1
• 氢受体数：
  1

反应信息

• 作为产物：
  描述：

  4,4'-联吡啶二硫醚 、 2-氨基乙基-三甲基铵 、 棕榈酰辅酶A,钾盐 在 N-terminally His₁₀-tagged human choline acetyltransferase E₃₃₇Y/C₅₅₀A mutant 作用下， 反应 0.5h， 生成 吡啶-4(1H)-硫酮 、 2-(Hexadecanoylamino)ethyl-trimethylazanium
  参考文献：
  名称：

  Redesign of Cosubstrate Specificity and Identification of Important Residues for Substrate Binding to hChAT

  摘要：

  In eukaryotes, choline acetyltransferase (ChAT) catalyzes the reversible formation of the neurotransmitter acetylcholine from choline and acetyl-CoA. ChAT belongs to a family of CoA-dependent enzymes that also includes the carnitine acyltransferases CrAT, CrOT, and CPTs. In contrast to CrOT and CPTs that are very active toward medium-and long-chain acyl-CoAs, respectively, CrAT and ChAT display activity toward only short-chain acyl-CoAs. We recently demonstrated the substrate and cosubstrate promiscuity of the wild-type human ChAT (hChAT). To extend the flexibility of this enzyme, we have generated a series of single, double, and triple hChAT mutants. Here we report the conversion of hChAT into choline octanoyltransferase (ChOT) and choline palmitoyltransferase (ChPT). The E337 and C550 residues (numbering from hChAT) were previously shown to dictate the acyl-CoA cosubstrate specificity in the carnitine series. Here we identify and demonstrate the importance of C551, in addition to E337 and C550, in contributing to the acyl-CoA specificity of hChAT. We also show that either C550 or C551 needs to be present for the transfer of medium-and long-chain acyl-CoAs by hChAT. By exploring the potential expansion of the tunnel on the substrate side, we demonstrate that residues M84, Y436, and Y552 play a critical role in binding and holding the choline substrate in the ChAT active site.

  DOI：

  10.1021/bi1007996

文献信息

• Degradable cationic surfactants and use thereof in enhancing chemiluminescence
  申请人：Siemens Healthcare Diagnostics Inc.

  公开号：US10859504B2

  公开（公告）日：2020-12-08

  The present invention relates to methods of enhancing chemiluminescence from a chemiluminescent label comprising contacting a chemiluminescent label with an acid in the presence of a degradable cationic surfactant and hydrogen peroxide followed by the addition of a base. Related kits containing such degradable cationic surfactant are also provided. The degradable cationic surfactant can compress light emission time of the chemiluminescent label to an extent that is comparable to or shorter than conventional surfactants. The degradable cationic surfactant can also increase chemiluminescence of the chemiluminescent label, providing increased light emission output that is comparable to conventional surfactants.

  本发明涉及增强化学发光标签化学发光的方法，包括在可降解阳离子表面活性剂和过氧化氢存在下，将化学发光标签与酸接触，然后加入碱。还提供了含有这种可降解阳离子表面活性剂的相关试剂盒。可降解阳离子表面活性剂可将化学发光标签的发光时间压缩到与传统表面活性剂相当或更短的程度。可降解阳离子表面活性剂还能增加化学发光标签的化学发光，提供与传统表面活性剂相当的更高的光发射输出。
• DEGRADABLE CATIONIC SURFACTANTS AND USE THEREOF IN ENHANCING CHEMILUMINESCENCE
  申请人：SIEMENS HEALTHCARE DIAGNOSTICS

  公开号：US20160131588A1

  公开（公告）日：2016-05-12

  The present invention relates to methods of enhancing chemiluminescence from a chemiluminescent label comprising contacting a chemiluminescent label with an acid in the presence of a degradable cationic surfactant and hydrogen peroxide followed by the addition of a base. Related kits containing such degradable cationic surfactant are also provided. The degradable cationic surfactant can compress light emission time of the chemiluminescent label to an extent that is comparable to or shorter than conventional surfactants. The degradable cationic surfactant can also increase chemiluminescence of the chemiluminescent label, providing increased light emission output that is comparable to conventional surfactants.