[EN] POLYNUCLEOTIDE SYNTHESIS METHOD, KIT AND SYSTEM<br/>[FR] PROCÉDÉ, KIT ET SYSTÈME DE SYNTHÈSE DE POLYNUCLÉOTIDES
申请人:OXFORD NANOPORE TECH LTD
公开号:WO2019150134A1
公开(公告)日:2019-08-08
The invention relates to new in vitro methods for synthesising a polymer, particularly a polynucleotide molecule, having a pre-defined sequence of units such as nucleotides. For synthesising a polynucleotide molecule the methods involve a process of extending a polynucleotide synthesis molecule with a transfer nucleotide. The methods additionally involve repeating the extension process multiple times to iteratively extend the polynucleotide molecule with multiple transfer nucleotides to generate a new polynucleotide molecule having a pre-defined nucleotide sequence. The invention also relates to in vitro methods of joining multiple synthetic polynucleotides following synthesis to form larger synthetic polynucleotides, as well as devices and systems for performing the extension, synthesis and assembly methods of the invention.