Ethyl 3-(2-hydroxyethoxy)-4-oxopentanoate

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 酮酸及其衍生物
• 英文名称: Ethyl 3-(2-hydroxyethoxy)-4-oxopentanoate
• 英文别名: ethyl 3-(2-hydroxyethoxy)-4-oxopentanoate
• 化学式: C₉H₁₆O₅
• 分子量: 204.22
• InChiKey: LEMZUJPZOHZUFH-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.6
• 重原子数：
  14
• 可旋转键数：
  8
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.78
• 拓扑面积：
  72.8
• 氢给体数：
  1
• 氢受体数：
  5

文献信息

• [EN] METHOD OF PRODUCING POLYHYDROXYALKANOATES (PHA) FROM OIL SUBSTRATE<br/>[FR] PROCÉDÉ DE PRODUCTION DE POLYHYDROXYALCANOATES (PHA) SUR LA BASE D'UN SUBSTRAT HUILEUX
  申请人：VUT V BRNE

  公开号：WO2014032633A1

  公开（公告）日：2014-03-06

  In the method of producing polyhydroxyalkanoates (PHAs) on an oil substrate according to the invention, whereby the oil substrate comprises vegetable oil and/or edible oil and/or waste edible oil, preferably frying oil, the bacterial strain Cupriavidus necator H16 is grown, converting oil into PHAs and at the same time producing its own extracellular lipolytic enzymes, which are at least partially isolated from the culture medium during the fermentation process before PHA production and isolation has completed. Lipolytic enzymes isolated from C.necator during the cultivation are used for the treatment of the oil substrate in such a manner that they are added to the production medium either before the beginning of the cultivation or at the beginning of the cultivation together with inoculum and in both cases also during the cultivation together with an additional dose of oil. In the isolation of PHAs produced by the method according to the invention, after fermentation has completed, the content of the production reactor is warmed up to the temperature of 80°C for at least 30 minutes, subsequently it is cooled to the temperature of about 60°C and lytic agents containing a mixture of a detergent, such as SDS, and alkase (proteolytic enzyme) are added, by which means raw homogenate, or lysate, is obtained. Another option is isolation of PHAs by means of organic solvents - dimethyl sulfoxide or acetone.