prop-2-yn-1-yl N-{2-[2-(2-aminoethoxy)ethoxy]ethyl} carbamate

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: prop-2-yn-1-yl N-{2-[2-(2-aminoethoxy)ethoxy]ethyl} carbamate
• 英文别名: prop-2-yn-1-yl N-{2-[2-(2-aminoethoxyl)ethoxy]ethyl}carbamate；2-Propyn-1-yl N-[2-[2-(2-aminoethoxy)ethoxy]ethyl]carbamate；prop-2-ynyl N-[2-[2-(2-aminoethoxy)ethoxy]ethyl]carbamate
• 化学式: C₁₀H₁₈N₂O₄
• 分子量: 230.264
• InChiKey: AQVBWOYGVFKQMD-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.2
• 重原子数：
  16
• 可旋转键数：
  10
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.7
• 拓扑面积：
  82.8
• 氢给体数：
  2
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-α-D-galactopyranosyl-(1→4)-6-O-acetyl-2,3-di-O-benzoyl-β-D-galactopyranosyl-(1→4)-3-O-acetyl-6-(4-nitrophenylcarbonate)-1,2-O-[(S)-1-(methoxycarbonyl)ethylidene]-α-D-glucopyranoside 、 prop-2-yn-1-yl N-{2-[2-(2-aminoethoxy)ethoxy]ethyl} carbamate 在 三乙胺 作用下， 以 二氯甲烷 为溶剂， 反应 2.0h， 以95%的产率得到2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-α-D-galactopyranosyl-(1→4)-6-O-acetyl-2,3-di-O-benzoyl-β-D-galactopyranosyl-(1→4)-3-O-acetyl-6-O-(10-oxo-3,6,11-trioxa-9-azatetradec-13-ynyl)carbamoyl-1,2-O-[(S)-1-(methoxycarbonyl)ethylidene]-α-D-glucopyranoside
  参考文献：
  名称：

  The synthesis of a multivalent heterobifunctional ligand for specific interaction with Shiga toxin 2 produced by E. coli O157:H7

  摘要：

  Hemolytic uremic syndrome is a potentially fatal complication of food poisoning caused by Escherichia coli O157:H7, especially those strains that produce the Stx2 Shiga toxin. Multivalent inhibitors based on the P-k trisaccharide are most effective against Stx1 the less dangerous of the two Shiga toxins. Inhibitors containing a terminal 2-acetamido-2-deoxy-alpha-D-galactopyranosyl residue in place of the terminal alpha-D-galactopyranosyl residue of P-k trisaccharide have been shown to exhibit preferential binding to Stx2. A multivalent heterobifunctional P-k analog containing 2-acetamido-2-deoxy-alpha-D-galactopyranose has been synthesized in a format that facilitates the ablation of toxin activity via supramolecular complex formation between Stx and the endogenous protein, Human serum amyloid P component (HuSAP). (C) 2013 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.carres.2013.05.010

文献信息

• Cell lines
  申请人：MEDIMMUNE LIMITED

  公开号：US10253345B2

  公开（公告）日：2019-04-09

  There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.

  除其他外，还提供了一种稳定真核细胞系的方法，该细胞系表达 PylRS 和 tRNAPyl，适于加入编码含有一个或多个由无意义密码子编码的非天然氨基酸的目标蛋白质的基因，该方法包括在减少或消除 tRNAPyl 表达对细胞活力和/或细胞生长的不利影响的条件下培养所述细胞系。
• US20150259721A1
  申请人：——

  公开号：US20150259721A1

  公开（公告）日：2015-09-17
• CELL LINES
  申请人：MEDIMMUNE LIMITED

  公开号：US20170306381A1

  公开（公告）日：2017-10-26

  There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.
• US9732367B2
  申请人：——

  公开号：US9732367B2

  公开（公告）日：2017-08-15
• [EN] CELL LINES<br/>[FR] LIGNÉE CELLULAIRE
  申请人：ALLOZYNE INC

  公开号：WO2014044872A1

  公开（公告）日：2014-03-27

  There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated.