2-Amino-6-[(2-methylpropan-2-yl)oxycarbonylamino]-5-sulfanylhexanoic acid

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 2-Amino-6-[(2-methylpropan-2-yl)oxycarbonylamino]-5-sulfanylhexanoic acid
• 英文别名: 2-amino-6-[(2-methylpropan-2-yl)oxycarbonylamino]-5-sulfanylhexanoic acid
• 化学式: C₁₁H₂₂N₂O₄S
• 分子量: 278.37
• InChiKey: CNXVYTJIEPFZNM-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -1.6
• 重原子数：
  18
• 可旋转键数：
  8
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.82
• 拓扑面积：
  103
• 氢给体数：
  4
• 氢受体数：
  6

文献信息

• Traceless Ubiquitination
  申请人：Chin Jason

  公开号：US20140148576A1

  公开（公告）日：2014-05-29

  The invention related to a tRNA synthetase capable of binding delta-substituted lysine, wherein said tRNA synthetase comprises amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS, wherein said sequence comprises 5 or fewer substitutions within the amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS; and wherein said synthetase comprises W at amino acid position 349 relative to MbPyIRS.

  该发明涉及一种能够结合δ-取代赖氨酸的tRNA合成酶，其中该tRNA合成酶包括与MbPyIRS的至少L271到Y349的氨基酸序列相对应的氨基酸序列，其中该序列在与MbPyIRS的至少L271到Y349的氨基酸序列相对应的氨基酸序列内仅包含5个或更少的替换；并且该合成酶在相对于MbPyIRS的氨基酸位置349处包含W。
• GENETICALLY EXPANDED CELL FREE PROTEIN SYNTHESIS SYSTEMS, METHODS AND KITS
  申请人：ALFONTA Lital

  公开号：US20170292139A1

  公开（公告）日：2017-10-12

  This invention relates to methods of producing a rare amino acid- or non-natural amino acid-containing protein in a cell free protein synthesis system and kits for use in and for accomplishing same. Specifically, the methods comprise the steps of expressing at least one orthogonal suppressor tRNA (o-tRNA)/aminoacyl-tRNA synthetase (aaRS) pair or derivatives thereof specific for incorporation of a rare amino acid- or non-natural amino acid in an E. coli organism; preparing a lysate of said E. coli organism expressing said orthogonal suppressor tRNA (o-tRNA)/aminoacyl-tRNA synthetase (aaRS) pair; and contacting said lysate with a template DNA containing a mutant gene in which at least one amino acid codon at a given site of the protein-encoding gene has been mutated into an amber or ochre mutation and further providing a cognate rare amino acid or non-natural amino acid and other factors necessary for protein synthesis; wherein protein synthesis occurs following said contact to produce a protein containing said at least one rare amino acid or said non-natural amino acid. Kits for use are described, as well.
• [EN] GENETICALLY EXPANDED CELL FREE PROTEIN SYNTHESIS SYSTEMS, METHODS AND KITS<br/>[FR] SYSTÈMES, PROCÉDÉS ET KITS DE SYNTHÈSE ACELLULAIRE PAR VOIE GÉNÉTIQUE D'UNE LARGE GAMME DE PROTÉINES
  申请人：B G NEGEV TECHNOLOGIES AND APPLICATIONS LTD AT BEN

  公开号：WO2015193897A1

  公开（公告）日：2015-12-23

  This invention relates to methods of producing a rare amino acid- or non-natural amino acid-containing protein in a cell free protein synthesis system and kits for use in and for accomplishing same. Specifically, the methods comprise the steps of expressing at least one orthogonal suppressor tRNA (o-tRNA)/aminoacyl-tRNA synthetase (aaRS) pair or derivatives thereof specific for incorporation of a rare amino acid- or non-natural amino acid in an E. coli organism; preparing a lysate of said E. coli organism expressing said orthogonal suppressor tRNA (o- tRNA)/aminoacyl-tRNA synthetase (aaRS) pair; and contacting said lysate with a template DNA containing a mutant gene in which at least one amino acid codon at a given site of the protein- encoding gene has been mutated into an amber or ochre mutation and further providing a cognate rare amino acid or non-natural amino acid and other factors necessary for protein synthesis; wherein protein synthesis occurs following said contact to produce a protein containing said at least one rare amino acid or said non-natural amino acid. Kits for use are described, as well.