A buffer kit includes a first eluent and second eluent. The first eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The second eluent solution includes at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge, and include a sulfonate group and an amine. The first eluent solution has a first pH and the second eluent solution has a second pH where the first pH and second pH are different values. The buffer kit provides a linear pH gradient that forms an approximately straight line from at least the first pH to the second pH.
缓冲液试剂盒包括第一洗脱液和第二洗脱液。第一洗脱液包括至少四种缓冲盐,其中至少三种缓冲盐是一价缓冲盐,具有净负电荷或净中性齐聚电荷,并包括
磺酸盐基团和胺。第二洗脱液包括至少四种缓冲盐,其中至少三种缓冲盐为一价缓冲盐,具有净负电荷或净中性齐聚电荷,并包括
磺酸基和胺。第一洗脱液具有第一 pH 值,第二洗脱液具有第二 pH 值,其中第一 pH 值和第二 pH 值为不同值。缓冲试剂盒提供线性 pH 梯度,至少从第一 pH 值到第二 pH 值形成近似直线。