QUANTITATIVE SOFT AGAR COLONY FORMATION ASSAY USING TETRAZOLIUM THAT GENERATES WATER-SOLUBLE FORMAZAN
申请人:KAGOSHIMA UNIVERSITY
公开号:US20140248655A1
公开(公告)日:2014-09-04
This invention provides a quantitative assay technique for soft agar colony formation that can be carried out rapidly with high accuracy. Specifically, this invention relates to a method for evaluation of cell survival comprising: a step of overlaying agar at the bottom of a vessel with agar containing cells, overlaying the agar containing cells with a medium and culturing the cells; a step of removing the medium, adding tetrazolium that produces water-soluble formazan and an electron carrier and culturing the cells; and a step of evaluating cell survival based on the color developed by the water-soluble formazan.