The present invention provides cationic lipids and lipid nanoparticle formulations comprising these lipids, alone or in combination with other lipids. These lipid nanoparticles may be formulated with nucleic acids to facilitate their intracellular delivery both in vitro and for therapeutic applications. The present invention also provides methods of chemical synthesis of these lipids, lipid nanoparticle preparation and formulation with nucleic acids.
本发明提供了阳离子脂质和脂质纳米粒子配方,包括这些脂质单独使用或与其他脂质结合。这些脂质纳米颗粒可以与核酸配方,以促进它们在体外和治疗应用中的细胞内传递。本发明还提供了这些脂质的
化学合成方法,脂质纳米颗粒制备和与核酸配方的配方方法。