The present invention relates to a microorganism of the genus Cupriavidus or Ralstonia, which is genetically modified to express phosphomannose isomerase (EC5.3.1.8) and facilitated diffusion protein for mannose uptake (EC1.3.1.74), and optionally mannofructokinase (EC2.7.1.4) and/or xylose isomerase (EC 5.3.1.5), xylulokinase (E 2.7.1.17) and xylose proton symporter E or a high affinity ABC- transporter. A modified Cupriavidus or Ralstonia host is capable of growing on mannose, xylose, arabinose, glucose, or galactose, or a combination thereof as the carbon source.
本发明涉及一种经转
基因表达
磷酸甘露糖异构酶(
EC5.3.1.8)和
甘露糖摄取促进扩散蛋白(
EC1.3.1.74)、
甘露糖激酶(
EC2.7.1.4)和/或
木糖异构酶(
EC 5.3.1.5)、
木糖激酶(E 2.7.1.17)和
木糖质子合流酶 E 或高亲和性 ABC 转运体。改良的
铜绿菌或拉氏菌宿主能够以
甘露糖、
木糖、
阿拉伯糖、
葡萄糖或半
乳糖或它们的组合作为碳源生长。