METHOD FOR QUANTITATIVELY DETERMINING CHOLESTEROL IN LOW-DENSITY LIPOPROTEINS, REAGENT FOR QUANTITATIVE DETERMINATION, AND QUANTITATIVE DETERMINATION KIT
申请人:Kyowa Medex CO., LTD.
公开号:EP2363498A1
公开(公告)日:2011-09-07
A method for measuring cholesterol in low-density lipoprotein contained in a sample, which comprises reacting a sample with (i) a combination of cholesterol ester hydrolase and cholesterol oxidase or (ii) a combination of cholesterol ester hydrolase, an oxidized coenzyme and cholesterol dehydrogenase in the presence of: [a] a polyoxyethylene-polyoxyalkylene alkylaryl ether; [b] one or more surfactants selected from the group consisting of a polyoxyethylene-polyoxyalkylene copolymer, a polyoxyethylene alkenyl ether, a polyoxyethylene branched alkyl ether, and a polyoxyethylene-polyoxyalkylene branched alkyl ether; [c] one or more surfactants selected from the group consisting of a primary amine, a secondary amine, a tertiary amine, and a quaternary ammonium; and [d] a polyanion, and measuring a substance formed or consumed in the reaction.
一种测量样品中所含低密度脂蛋白中胆固醇的方法,它包括在下列物质存在的情况下,使样品与(i)胆固醇酯水解酶和胆固醇氧化酶的组合或(ii)胆固醇酯水解酶、氧化辅酶和胆固醇脱氢酶的组合发生反应:[a] 聚氧乙烯-聚氧亚烷基芳基醚; [b] 一种或多种表面活性剂,选自由聚氧乙烯-聚氧亚烷基共聚物、聚氧乙烯烯基醚、聚氧乙烯支链烷基醚和聚氧乙烯-聚氧亚烷基支链烷基醚组成的组;[c] 一种或多种表面活性剂,选自由伯胺、仲胺、叔胺和季铵组成的组;以及 [d] 一种聚阴离子,并测量反应中形成或消耗的物质。