6-碘乙酰氨基荧光素 | 73264-12-7

• 物质功能分类: 生物化工 - 生化试剂 - 荧光成像
• 分子结构分类: 有机化合物 - 有机杂环化合物 - 苯并吡喃
• 中文名称: 6-碘乙酰氨基荧光素
• 英文名称: 6-Iodoacetamidofluorescein
• 英文别名: N-(3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-yl)-2-iodoacetamide
• CAS: 73264-12-7
• 化学式: C₂₂H₁₄INO₆
• mdl: MFCD00155654
• 分子量: 515.3
• InChiKey: YRDPEKZBFANDFE-UHFFFAOYSA-N

物化性质

• 沸点：
  798.5±60.0 °C(Predicted)
• 密度：
  1.95±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  3.3
• 重原子数：
  30
• 可旋转键数：
  2
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.09
• 拓扑面积：
  105
• 氢给体数：
  3
• 氢受体数：
  6

安全信息

• WGK Germany：
  3

制备方法与用途

生物活性：6-碘乙酰氨基荧光素是一种巯基特异性的荧光染料，可用于选择性地标记核基质多肽和蛋白质中的 -SH 基团。

文献信息

• Novel sulfonamides as L-CPT1 inhibitors
  申请人：Bleicher Konrad

  公开号：US20060276494A1

  公开（公告）日：2006-12-07

  The invention is concerned with novel sulfonamide derivatives of formula (I) wherein R 2 , R 3 , R 4 , A, X, Y 1 , Y 2 , Y 3 , Y 4 and Z 1 are as defined in the description and in the claims, as well as physiologically acceptable salts and esters thereof. These compounds inhibit L-CPT1 and can be used as medicaments.

  这项发明涉及式(I)的新型磺胺衍生物， 其中R 2 ，R 3 ，R 4 ，A，X，Y 1 ，Y 2 ，Y 3 ，Y 4 和Z 1 如描述和索赔中所定义，并且其生理上可接受的盐和酯。这些化合物抑制L-CPT1，可用作药物。
• Multiplex analysis using membrane-bound sensitizers
  申请人：——

  公开号：US20030170915A1

  公开（公告）日：2003-09-11

  The present invention is directed to methods and compositions for determining the presence, absence, and/or amounts of one or more membrane-associated analytes in a sample. In accordance with the invention, binding compounds derivatized with releasable molecular tags specifically bind to selected membrane-associated analytes, after which the molecular tags are released upon activation of cleavage moieties, or sensitizers, anchored in the same membrane as the membrane-associated analytes. The released molecular tags are then identified by their distinct separation and detection characteristics.

  本发明涉及一种用于确定样品中一个或多个膜相关分析物的存在、缺失和/或数量的方法和组合物。根据本发明，与可释放分子标签衍生化的结合化合物特异性地结合选择的膜相关分析物，之后在与膜相关分析物锚定在同一膜中的裂解基团或敏化剂的激活下，分子标签被释放。然后，通过其独特的分离和检测特性识别释放的分子标签。
• Methods and compositions for analyzing proteins
  申请人：——

  公开号：US20030013126A1

  公开（公告）日：2003-01-16

  Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and a first binding agent for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of post-translational modification activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in the post-translational modification of the target polypeptide. The interaction between the first binding agent and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags.

  本发明揭示了一种用于确定样品中经历了翻译后修饰的一个或多个目标多肽的方法、组合物和试剂盒。将包含样品和第一试剂的混合物暴露在相应的结合物质发生结合的条件下，其中第一试剂包括一个裂解诱导基团和一个与目标多肽上的结合位点结合的第一结合剂。该结合位点是涉及目标多肽的翻译后修饰活性的结果。该方法可用于确定目标多肽本身。在另一实施例中，目标多肽的存在和/或数量与参与目标多肽翻译后修饰的酶等试剂的存在、数量和/或活性相关。第一结合剂和结合位点之间的相互作用将裂解诱导基团带到可裂解基团的近距离位置，后者与多肽相关，并且只有在接近裂解诱导基团时才易于裂解。通过这种方式，每个多肽的电泳标签可以被释放。释放的电泳标签被分离，并根据相应的电泳标签确定目标多肽的存在和/或数量。
• Multiplex analytical platform using molecular tags
  申请人：——

  公开号：US20030207300A1

  公开（公告）日：2003-11-06

  Compositions and methods are disclosed for detecting multiple target analytes, particularly polynucleotide target analytes. In accordance with one aspect of the invention, a template-dependent extension reaction is performed to generate detection probes, such that each detection probe has (i) at least one molecular tag attached by a cleavable linkage and (ii) either a capture moiety or a cleavage-inducing moiety attached. The template-dependent extension reaction may be carried out directly on a polynucleotide analyte to generate molecular tags, wherein the polynucleotide analyte serves as a template in the template-dependent extension reaction, or it may be carried out indirectly on an oligonucleotide label that, in turn, is attached to a binding moiety specific for an analyte of interest. In either case, a plurality of molecular tags are generated, after which they are separated and identified to determine the presence or absence or the quantity of the target analytes in a sample.

  本发明揭示了检测多种目标分析物的组合物和方法，特别是聚核苷酸目标分析物。根据本发明的一个方面，执行基于模板的延伸反应来生成检测探针，使得每个检测探针具有（i）至少一个通过可断裂的连接物连接的分子标签和（ii）连接了捕获基团或诱导断裂基团中的一种。基于模板的延伸反应可以直接在聚核苷酸分析物上进行，以生成分子标签，其中聚核苷酸分析物在基于模板的延伸反应中作为模板，或者间接地在寡核苷酸标签上进行，寡核苷酸标签反过来附着在特定于感兴趣的分析物的结合基团上。在任一情况下，生成多个分子标签，然后将它们分离和鉴定，以确定样品中目标分析物的存在或不存在或数量。
• Analyzing phosphorylated proteins
  申请人：——

  公开号：US20030040016A1

  公开（公告）日：2003-02-27

  Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone phosphorylation. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and an IMAC resin for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of phosphorylation activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in phosphorylation of the target polypeptide. The interaction between the IMAC resin and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags.

  本发明揭示了一种用于确定样品中已经磷酸化的一个或多个目标多肽的方法、组合物和试剂盒。将包含样品和第一试剂的混合物，第一试剂包含一个裂解诱导基团和一个IMAC树脂，用于目标多肽上的结合位点，置于结合各自结合基团的条件下。结合位点是涉及目标多肽的磷酸化活性的结果。该方法可用于确定目标多肽本身。在另一实施例中，目标多肽的存在和/或量与参与目标多肽磷酸化的酶等试剂的存在和/或量和/或活性有关。IMAC树脂与结合位点之间的相互作用将裂解诱导基团带到可裂解基团附近，该可裂解基团与多肽相关，并且仅在与裂解诱导基团接近时易于裂解。通过这种方式，可以释放每个多肽的电泳标记。释放的电泳标记被分离，并根据相应的电泳标记确定目标多肽的存在和/或量。