N-acetylglutamyl 5-phosphate | 15383-57-0

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: N-acetylglutamyl 5-phosphate
• 英文别名: N-acetyl-γ-glutamyl phosphate；N-Acetyl-L-glutamyl 5-phosphate；(2S)-2-acetamido-5-oxo-5-phosphonooxypentanoic acid
• CAS: 15383-57-0
• 化学式: C₇H₁₂NO₈P
• 分子量: 269.148
• InChiKey: FCVIHFVSXHOPSW-YFKPBYRVSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -2.3
• 重原子数：
  17
• 可旋转键数：
  7
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.57
• 拓扑面积：
  150
• 氢给体数：
  4
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  N-acetylglutamyl 5-phosphate 在 盐酸羟胺 作用下， 生成 N-acetyl-L-Glu-γ-mono-N-hydroxyamide
  参考文献：
  名称：

  Probing the Role of N-Acetyl-glutamyl 5-Phosphate, an Acyl Phosphate, in the Construction of the Azabicycle Moiety of the Azinomycins

  摘要：

  阿齐霉素是由土壤细菌Streptomyces sahachiroi产生的强效抗肿瘤药物，含有新型氮丙啶[1,2-a]吡咯烷核心，其合成至少涉及14个步骤。本研究首次报道了由阿齐霉素生物合成基因簇编码的两个酶对N-乙酰谷氨酰胺半醛形成的重组。该反应通过形成酰基磷酸，并建立N-乙酰谷氨酰5-磷酸和N-乙酰谷氨酰胺半醛作为氮丙啶[1,2-a]吡咯烷部分复杂生物合成的中间体。

  DOI：

  10.1021/acs.biochem.5b00711
• 作为产物：
  描述：

  N-乙酰-L-谷氨酸 在 腺苷 5'-三磷酸酯-gamma-32P 作用下， 反应 16.0h， 生成 N-acetylglutamyl 5-phosphate
  参考文献：
  名称：

  Probing the Role of N-Acetyl-glutamyl 5-Phosphate, an Acyl Phosphate, in the Construction of the Azabicycle Moiety of the Azinomycins

  摘要：

  阿齐霉素是由土壤细菌Streptomyces sahachiroi产生的强效抗肿瘤药物，含有新型氮丙啶[1,2-a]吡咯烷核心，其合成至少涉及14个步骤。本研究首次报道了由阿齐霉素生物合成基因簇编码的两个酶对N-乙酰谷氨酰胺半醛形成的重组。该反应通过形成酰基磷酸，并建立N-乙酰谷氨酰5-磷酸和N-乙酰谷氨酰胺半醛作为氮丙啶[1,2-a]吡咯烷部分复杂生物合成的中间体。

  DOI：

  10.1021/acs.biochem.5b00711

文献信息

• Probing the Role of <i>N</i>-Acetyl-glutamyl 5-Phosphate, an Acyl Phosphate, in the Construction of the Azabicycle Moiety of the Azinomycins
  作者：Keshav K. Nepal、Rachel P. Lee、Yohannes H. Rezenom、Coran M. H. Watanabe

  DOI：10.1021/acs.biochem.5b00711

  日期：2015.7.28

  The azinomycins are potent antitumor agents produced by the soil bacterium Streptomyces sahachiroi and contain a novel aziridino[1,2-a]pyrrolidine core; its synthesis involves at least 14 steps. This study reports the first reconstitution of N-acetylglutamine semialdehyde formation by two enzymes encoded in the azinomycin biosynthetic gene cluster. The reaction proceeds through the formation of an acylphosphate and establishes N-acetyl-glutamyl 5-phosphate and N-acetylglutamine semialdehyde as intermediates in the complex biosynthesis of the aziridino[1,2-a]pyrrolidine moiety.

  阿齐霉素是由土壤细菌Streptomyces sahachiroi产生的强效抗肿瘤药物，含有新型氮丙啶[1,2-a]吡咯烷核心，其合成至少涉及14个步骤。本研究首次报道了由阿齐霉素生物合成基因簇编码的两个酶对N-乙酰谷氨酰胺半醛形成的重组。该反应通过形成酰基磷酸，并建立N-乙酰谷氨酰5-磷酸和N-乙酰谷氨酰胺半醛作为氮丙啶[1,2-a]吡咯烷部分复杂生物合成的中间体。
• Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
  申请人：Ceres Incorporated

  公开号：EP1033405A2

  公开（公告）日：2000-09-06

  The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3' termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait. 0Arabidopsis DNA is used in the present experiment, but the procedure is a general one.

  本发明提供了构成植物基因组片段的 DNA 分子及其编码的多肽。这些 DNA 分子可作为启动子或蛋白质编码序列或 UTR 或 3'终止序列，用于指定细胞中的基因产物，也可用于控制染色体中基因的行为，控制基因的表达，或作为基因绘图、识别或分离相同或相关 DNA 片段、或识别特定生物个体、或对具有共同性状的生物群体进行聚类的工具。 本实验中使用的是拟南芥 DNA，但这是一个通用程序。
• DNA Sequences
  申请人：Ceres Incorporated

  公开号：EP1887081A2

  公开（公告）日：2008-02-13

  The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3' termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait.

  本发明提供了构成植物基因组片段的 DNA 分子及其编码的多肽。这些 DNA 分子可作为启动子或蛋白质编码序列或 UTR 或 3'终止序列，用于指定细胞中的基因产物，也可用于控制染色体中基因的行为，控制基因的表达，或作为基因绘图、识别或分离相同或相关 DNA 片段、或识别特定生物个体或具有共同性状的生物群体的工具。
• Ornithine biosynthesis enzymes
  申请人：——

  公开号：US20030093827A1

  公开（公告）日：2003-05-15

  This invention relates to an isolated nucleic acid fragment encoding an ornithine biosynthetic enzyme, more specifically an ornithine acetyltransferase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the ornithine acetyltransferase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the ornithine acetyltransferase in a transformed host cell.

  本发明涉及一种编码鸟氨酸生物合成酶，更具体地说是鸟氨酸乙酰转移酶的分离核酸片段。本发明还涉及以有义或反义方向构建编码全部或部分鸟氨酸乙酰转移酶的重组 DNA 构建体，其中重组 DNA 构建体的表达可导致转化宿主细胞中鸟氨酸乙酰转移酶水平的改变。
• Methods for conducting assays for enzyme activity on protein microarrays
  申请人：Zhou X. Fang

  公开号：US20050118665A1

  公开（公告）日：2005-06-02

  The present invention relates to methods of conducting assays for enzymatic activity on microarrays useful for the large-scale study of protein function, screening assays, and high-throughput analysis of enzymatic reactions. The invention relates to methods of using protein chips to assay the presence, amount, activity and/or function of enzymes present in a protein sample on a protein chip. In particular, the methods of the invention relate to conducting enzymatic assays using a microarray wherein a protein and a substance are immobilized on the surface of a solid support and wherein the protein and the substance are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the substance and the protein. The invention also relates to microarrays that have an enzyme and a substrate immobilized on their surface wherein the enzyme and the substrate are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the enzyme and the substrate.

  本发明涉及在微阵列上进行酶活性测定的方法，这种方法可用于蛋白质功能的大规模研究、筛选测定和酶反应的高通量分析。本发明涉及使用蛋白质芯片检测蛋白质芯片上蛋白质样品中酶的存在、数量、活性和/或功能的方法。特别是，本发明的方法涉及使用芯片进行酶测定，其中蛋白质和物质被固定在固体支持物表面，蛋白质和物质相互靠近，足以使物质和蛋白质之间发生酶反应。本发明还涉及在其表面固定有酶和底物的微阵列，其中酶和底物相互靠近，足以使酶和底物之间发生酶反应。