3-methyl-2,3-dihydro-1H-pyrrole-2-carboxylic acid

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: 3-methyl-2,3-dihydro-1H-pyrrole-2-carboxylic acid
• 化学式: C₆H₉NO₂
• 分子量: 127.14
• InChiKey: ZVJPMCWYCLEWPG-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.8
• 重原子数：
  9
• 可旋转键数：
  1
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  49.3
• 氢给体数：
  2
• 氢受体数：
  3

文献信息

• [EN] BIOSYNTHETICALLY GENERATED PYRROLINE-CARBOXY-LYSINE AND SITE SPECIFIC PROTEIN MODIFICATIONS VIA CHEMICAL DERIVATIZATION OF PYRROLINE-CARBOXY-LYSINE AND PYRROLYSINE RESIDUES<br/>[FR] PYRROLINE-CARBOXY-LYSINE PRODUITE PAR BIOSYNTHÈSE ET MODIFICATIONS DE PROTÉINES SPÉCIFIQUES DE SITE OBTENUES PAR UNE DÉRIVATISATION CHIMIQUE DE PYRROLINE-CARBOXY-LYSINE ET DE RÉSIDUS DE PYRROLYSINE
  申请人：IRM LLC

  公开号：WO2010048582A1

  公开（公告）日：2010-04-29

  Disclosed herein is pyrroline-carboxy-lysine (PCL), a pyrrolysine analogue, which is a natural, biosynthetically generated amino acid, and methods for biosynthetically generating PCL. Also disclosed herein are proteins, polypeptides and peptides that have PCL incorporated therein and methods for incorporating PCL into such proteins, polypeptides and peptides. Also disclosed herein is the site-specific derivatization of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein. Also disclosed herein is the crosslinking of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein.

  本文披露了吡咯酮羧基赖氨酸（PCL），一种吡咯赖氨酸类似物，它是一种天然的、生物合成产生的氨基酸，以及生物合成生成PCL的方法。本文还披露了含有PCL的蛋白质、多肽和肽以及将PCL纳入这些蛋白质、多肽和肽中的方法。本文还披露了具有PCL或吡咯赖氨酸的蛋白质、多肽和肽的特定位点衍生化。本文还披露了具有PCL或吡咯赖氨酸的蛋白质、多肽和肽的交联。
• Pyrrolysine synthesis and modification
  申请人：Chan K. Michael

  公开号：US20060183888A1

  公开（公告）日：2006-08-17

  Synthetic L-pyrrolysine of formula I, and derivatives thereof, chemical methods of preparing these amino acids and derivatives, and methods of derivatizing these amino acids after incorporation into a peptide or protein. Also provided are derivatives and methods of preparing derivatives, including addition of substituents at any substitutable position; modifications to the lysine alkyl chain; modifications of the pyrroline ring. Also provided are methods of adding labels to the pyrrolysine or derivative thereof.

  提供公式I的合成L-吡咯氨酸及其衍生物，制备这些氨基酸和衍生物的化学方法，以及将这些氨基酸并入肽或蛋白质后衍生化的方法。还提供了衍生物和制备衍生物的方法，包括在任何可取代位置添加取代基；改变赖氨酸烷基链的结构；改变吡咯环的结构。还提供了将标签添加到吡咯氨酸或其衍生物的方法。
• BIOSYNTHETICALLY GENERATED PYRROLINE-CARBOXY-LYSINE AND SITE SPECIFIC PROTEIN MODIFICATIONS VIA CHEMICAL DERIVATIZATION OF PYRROLINE-CARBOXY-LYSINE AND PYRROLYSINE RESIDUES
  申请人：Geierstanger Bernhard

  公开号：US20110262963A1

  公开（公告）日：2011-10-27

  Disclosed herein is pyrroline-carboxy-lysine (PCL), a pyrrolysine analogue, which is a natural, biosynthetically generated amino acid, and methods for biosynthetically generating PCL. Also disclosed herein are proteins, polypeptides and peptides that have PCL incorporated therein and methods for incorporating PCL into such proteins, polypeptides and peptides. Also disclosed herein is the site-specific derivatization of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein. Also disclosed herein is the crosslinking of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein.

  本文披露了一种吡咯赖氨酸（PCL），它是一种天然的类似吡咯赖氨酸的氨基酸，通过生物合成生成，并且揭示了生物合成生成PCL的方法。本文还披露了具有PCL的蛋白质、多肽和肽段，以及将PCL纳入这些蛋白质、多肽和肽段中的方法。本文还披露了具有PCL或吡咯赖氨酸的蛋白质、多肽和肽段的位点特异性衍生化。本文还披露了具有PCL或吡咯赖氨酸的蛋白质、多肽和肽段的交联。
• Advanced drug development and manufacturing
  申请人：Los Alamos National Security, LLC

  公开号：EP2511844A2

  公开（公告）日：2012-10-17

  There is described an apparatus for measuring protein characteristics comprising an X-ray fluorescence (XRF) spectrometer comprising a source of polychromatic X-rays, an X-ray detector, a protein, a molecule that has been exposed to and at least weakly binds to the protein, a plurality of X-ray fluorescence signal data obtained by irradiating chemical elements in the protein and molecule with the polychromatic X-rays and a security system for maintaining records for the data from the plurality of X-ray fluorescence signal measurements. There is also described an x-ray microscope for measuring a sample.

  描述了一种测量蛋白质特性的仪器，该仪器包括一个 X 射线荧光 (XRF) 光谱仪，其中包括一个多色 X 射线源、一个 X 射线探测器、一个蛋白质、一个已暴露于该蛋白质并至少与该蛋白质弱结合的分子、通过用多色 X 射线照射蛋白质和分子中的化学元素而获得的多个 X 射线荧光信号数据，以及一个用于维护多个 X 射线荧光信号测量数据记录的安全系统。此外，还介绍了一种用于测量样品的 X 射线显微镜。
• Charging tRNA with pyrrolysine
  申请人：Chan K. Michael

  公开号：US20060166319A1

  公开（公告）日：2006-07-27

  Provided herein is a method for preparing a cell that, when exposed to the non-canonical amino acid, pyrrolysine or a derivative thereof and transformed or transfected with a polynucleotide comprising an in-frame UAG or TAG codon incorporates the pyrrolysine residue or derivative thereof into the protein or polynucleotide into the protein or polypeptide encoded by the polynucleotide. The method comprises introducing a first polynucleotide comprising a sequence that encodes a protein having pyrrolysyl-tRNA synthetase activity and a second polynucleotide comprising a sequence that encodes a pyrrolysine specific tRNA into the cell and maintining the cell under conditions that permit expression of the first and second polynucleotide. Also provided herein are modified cells produced in accordance with the present method and methods of making peptides, polypeptides, and proteins which utilize such modified cells. Also provide are kits comprising pyrrolysine or a pyrrolysine or both. The kits also provide the first polynucleotide and the second polynucelotide or cells produced in accordance with the present methods or both.

  本发明提供了一种制备细胞的方法，当细胞暴露于非典型氨基酸吡咯赖氨酸或其衍生物并用包含框架内UAG或TAG密码子的多核苷酸转化或转染时，该细胞将吡咯赖氨酸残基或其衍生物结合到蛋白质或多核苷酸中，结合到多核苷酸编码的蛋白质或多肽中。该方法包括将包含编码具有吡咯赖氨酸-tRNA 合成酶活性的蛋白质的序列的第一多核苷酸和包含编码吡咯赖氨酸特异性 tRNA 的序列的第二多核苷酸导入细胞，并在允许第一和第二多核苷酸表达的条件下维持细胞。本文还提供了按照本方法生产的修饰细胞，以及利用这种修饰细胞制造肽、多肽和蛋白质的方法。还提供了包含吡咯烷酮或吡咯烷酮或两者的试剂盒。这些试剂盒还提供第一种多核苷酸和第二种多核苷酸或按照本方法生产的细胞或两者。