申请人:{hacek over (S)}iurkus Juozas
公开号:US10196666B2
公开(公告)日:2019-02-05
A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells.
一种从宿主细胞中生产包含至少一个还原态半胱
氨酸残基的异源
多肽或蛋白质的方法。该方法在含有还原剂的
培养基中培养宿主细胞;并从宿主细胞或
培养基中回收包含至少一个还原态半胱
氨酸残基的异源
多肽或蛋白质,其中宿主细胞包含编码异源蛋白质或
多肽的核酸,还原剂能够渗透宿主细胞的质膜。