2'-Deoxy-5-hydroxymethylcytidine-5'-diphosphate | 94714-46-2

• 分子结构分类: 有机化合物 - 有机氧化合物 - 有机含氧阴离子化合物
• 英文名称: 2'-Deoxy-5-hydroxymethylcytidine-5'-diphosphate
• 英文别名: [(2R,3S,5R)-5-[4-amino-5-(hydroxymethyl)-2-oxopyrimidin-1-yl]-3-hydroxyoxolan-2-yl]methyl phosphono hydrogen phosphate
• CAS: 94714-46-2
• 化学式: C₁₀H₁₇N₃O₁₁P₂
• 分子量: 417.2
• InChiKey: RQKDPSTWKKMBPM-XLPZGREQSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -4.7
• 重原子数：
  26
• 可旋转键数：
  7
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.6
• 拓扑面积：
  222
• 氢给体数：
  6
• 氢受体数：
  11

文献信息

• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• BARCODING TECHNOLOGY FOR SEQUENCING OF NUCLEIC ACID MOLECULES
  申请人：UMC Utrecht Holding B.V.

  公开号：EP3878972A1

  公开（公告）日：2021-09-15

  The invention relates to barcodes that have both unique and shared sequences. The barcodes are easy to sequence and to identify. In one aspect the invention provides a collection of barcoded nucleic acid molecules wherein each barcode comprises a sequence that is shared with the other barcodes in the collection and a sequence that is not shared with the other barcodes in the collection wherein the nucleotide residues that encode the non-shared sequence are interspersed with the nucleotide residues that encode the shared sequence. The invention also provides kits and method for sequencing nucleic acids.

  本发明涉及具有唯一序列和共享序列的条形码。这些条形码易于测序和识别。在一个方面，本发明提供了一个条形码核酸分子集合，其中每个条形码包括一个与集合中其他条形码共享的序列和一个与集合中其他条形码不共享的序列，其中编码不共享序列的核苷酸残基与编码共享序列的核苷酸残基穿插在一起。本发明还提供了用于核酸测序的试剂盒和方法。
• Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
  申请人：Oxford Nanopore Technologies Ltd.

  公开号：US10597713B2

  公开（公告）日：2020-03-24

  The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.

  本发明涉及一种对双链目标多核苷酸进行测序的新方法。双链目标多核苷酸的两条链由桥接分子连接。利用多核苷酸结合蛋白将目标多核苷酸的两条链分开，并利用跨膜孔对目标多核苷酸进行测序。
• Method of analyzing post-translational modifications
  申请人：OXFORD UNIVERSITY INNOVATION LIMITED

  公开号：US10712254B2

  公开（公告）日：2020-07-14

  The invention relates to a new method of determining the presence, absence, number or position(s) of one or more post-translational modifications in a peptide, polypeptide or protein. The invention uses transmembrane pores.

  本发明涉及一种确定肽、多肽或蛋白质中一种或多种翻译后修饰存在、不存在、数量或位置的新方法。本发明使用跨膜孔。
• Aptamer method
  申请人：OXFORD NANOPORE TECHNOLOGIES LIMITED

  公开号：US10739341B2

  公开（公告）日：2020-08-11

  The invention relates to a new method of determining in a sample the presence or absence of one or more analyte members of a group of two or more analytes. The invention therefore relates to a multiplex assay for determining the presence or absence of each analyte in a group of multiple analytes. The assay uses aptamers and transmembrane pores.

  本发明涉及一种新方法，用于确定样品中是否存在由两种或两种以上分析物组成的一组分析物中的一种或多种分析物。因此，本发明涉及一种多重测定法，用于确定多个分析物群中每个分析物的存在或不存在。该检测方法使用了适配体和跨膜孔。