Citramalate

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 英文名称: Citramalate
• 英文别名: 2-hydroxy-2-methylbutanedioate
• 化学式: C5H6O5-2
• 分子量: 146.1
• InChiKey: XFTRTWQBIOMVPK-UHFFFAOYSA-L

计算性质

• 辛醇/水分配系数(LogP)：
  0.2
• 重原子数：
  10
• 可旋转键数：
  1
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.6
• 拓扑面积：
  101
• 氢给体数：
  1
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  Citramalate 、 苹果酸 生成 衣康酸
  参考文献：
  名称：

  Process for decreasing verbascose in a plant by expression of a chloroplast-targeted fimD protein

  摘要：

  本发明涉及一种通过在植物细胞、植物或其部分中表达靶向叶绿体的fimD超家族多肽来减少植物中verbascose的过程。本发明还涉及一种制备具有verbascose含量降低的植物细胞、植物或其部分的过程。还提供了用于实施该方法的核酸构建和载体，以及由此获得的植物、植物组织、繁殖材料和收获材料。还提供了包含这些植物材料的农业组合物。

  公开号：

  US08952217B2

文献信息

• Process for the production of fine chemicals
  申请人：Puzio Piotr

  公开号：US20070118916A1

  公开（公告）日：2007-05-24

  The present invention relates to a process for the production of the fine chemical in a microorganism, a plant cell, a plant, a plant tissue or in one or more parts thereof, preferably in plastids. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.

  本发明涉及一种在微生物、植物细胞、植物、植物组织或其一部分中生产精细化学品的过程，优选在质体中进行。此外，本发明还涉及核酸分子、多肽、核酸构建物、载体、抗体、宿主细胞、植物组织、繁殖材料、收获材料、植物、微生物以及农业组合物以及它们的用途。
• Isolation and Function of the Subunits of Citramalate Lyase and Formation of Hybrids with the Subunits of Citrate Lyase
  作者：Peter DIMROTH、Wolfgang BUCKEL、Rosemarie LOYAL、Hermann EGGERER

  DOI：10.1111/j.1432-1033.1977.tb11902.x

  日期：1977.11

  1. The enzyme complex citramalate lyase from Clostridium tetanomorphum, composed of six copies of three different proteins, has been dissociated into its protein components and these were isolated in an enzymically active state. Citramalate lyase has been reconstituted from the isolated subunits.2. The enzyme complex consists of an acyl carrier protein and two different enzymes, the α and β subunits. The isolated enzymes were active if acetyl‐thio‐acyl carrier protein and (3S)‐citramalyl‐thio‐acyl carrier protein were substituted for the corresponding acyl‐CoA derivatives.3. The α subunit in the presence of (3S)‐citramalyl‐CoA and acetate catalyzed the formation of acetyl‐CoA and citramalate. The β subunit catalyzed the cleavage of (3S)‐citramalyl‐CoA to acetyl‐CoA and pyruvate. This reaction was dependent on Mg2+ and was abolished if EDTA was added in excess. It was concluded from these results that the α subunit represents an acetyl‐thio‐acyl carrier protein: citramalate acyl carrier protein transferase within the native complex, and the β subunit a (3S)‐citramalyl‐thio‐acyl carrier protein lyase.4. Results similar to those given above have previously been obtained with the enzyme complex citrate lyase from Klebsiella aerogenes, but with citrate, (3S)‐citryl‐thio‐acyl carrier protein or (3S)‐citryl‐CoA instead of citramalate and its corresponding derivatives. Thus, not only molecular weight, subunit composition and chemical events during substrate turnover are nearly identical in the two different enzyme complexes but also the functions of the individual subunits.5. The close relationship between the two different enzymes from the two different microorganisms was confirmed by cross reactions between the isolated subunits of each enzyme complex producing a hybrid enzyme complex.
• Process for decreasing verbascose in a plant by expression of a chloroplast-targeted fimD protein
  申请人：Puzio Piotr

  公开号：US08952217B2

  公开（公告）日：2015-02-10

  The present invention relates to a process for decreasing verbascose in a plant by expression of a chloroplast-targeted polypeptide which is a member of the fimD superfamily in a plant cell, plant, or a part thereof. The invention furthermore relates to a process for producing a plant cell, plant, or part thereof with a decrease in the amount of verbascose. Also provided are nucleic acid constructs and vectors useful for practicing the methods as well as plants, plant tissues, propagation materials and harvested materials thus obtained. Agricultural compositions comprising the plant materials thus obtained is also provided.

  本发明涉及一种通过在植物细胞、植物或其部分中表达靶向叶绿体的fimD超家族多肽来减少植物中verbascose的过程。本发明还涉及一种制备具有verbascose含量降低的植物细胞、植物或其部分的过程。还提供了用于实施该方法的核酸构建和载体，以及由此获得的植物、植物组织、繁殖材料和收获材料。还提供了包含这些植物材料的农业组合物。