N-亚胺代甲酰基-L-谷氨酸 | 816-90-0

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: N-亚胺代甲酰基-L-谷氨酸
• 英文名称: N-formimidoyl-L-glutamic acid
• 英文别名: N-Formimidoyl-L-glutaminsaeure；N-(iminomethyl)-L-glutamic acid；N-Formimino-L-glutamat；Formiminoglutamic acid；(2S)-2-(aminomethylideneamino)pentanedioic acid
• CAS: 816-90-0
• 化学式: C₆H₁₀N₂O₄
• 分子量: 174.156
• InChiKey: NRXIKWMTVXPVEF-BYPYZUCNSA-N

物化性质

• 熔点：
  >161°C (dec.)
• 溶解度：
  酸水溶液（微溶，超声处理），水（微溶）
• 沸点：
  363.8±52.0 °C(Predicted)
• 密度：
  1.45±0.1 g/cm3(Predicted)
• 物理描述：
  Solid

计算性质

• 辛醇/水分配系数(LogP)：
  -1.1
• 重原子数：
  12
• 可旋转键数：
  5
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  113
• 氢给体数：
  3
• 氢受体数：
  5

安全信息

• 危险品标志：
  Xn
• 安全说明：
  S36
• 危险类别码：
  R20/21/22

反应信息

• 作为反应物：
  描述：

  tetrahydrofolic acid 、 N-亚胺代甲酰基-L-谷氨酸 生成 N-((S)-5-formimidoyl-5,6,7,8-tetrahydro-pteroyl)-L-glutamic acid
  参考文献：
  名称：

  INTERMEDIATE STEPS IN THE FORMYLATION OF TETRAHYDROFOLIC ACID BY FORMIMINOGLUTAMIC ACID IN RABBIT LIVER

  摘要：

  DOI：

  10.1021/ja01602a075
• 作为产物：
  描述：

  L-谷氨酸 γ-苄酯 在 甲醇 、 钯 、 formamide 、 silver carbonate 作用下， 生成 N-亚胺代甲酰基-L-谷氨酸
  参考文献：
  名称：

  α-L-Formamidinoglutaric Acid, an Intermediate in Histidine Metabolism¹

  摘要：

  DOI：

  10.1021/ja01652a101

文献信息

• Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
  申请人：Ceres Incorporated

  公开号：EP1033405A2

  公开（公告）日：2000-09-06

  The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3' termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait. 0Arabidopsis DNA is used in the present experiment, but the procedure is a general one.

  本发明提供了构成植物基因组片段的 DNA 分子及其编码的多肽。这些 DNA 分子可作为启动子或蛋白质编码序列或 UTR 或 3'终止序列，用于指定细胞中的基因产物，也可用于控制染色体中基因的行为，控制基因的表达，或作为基因绘图、识别或分离相同或相关 DNA 片段、或识别特定生物个体、或对具有共同性状的生物群体进行聚类的工具。 本实验中使用的是拟南芥 DNA，但这是一个通用程序。
• DNA Sequences
  申请人：Ceres Incorporated

  公开号：EP1887081A2

  公开（公告）日：2008-02-13

  The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3' termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait.

  本发明提供了构成植物基因组片段的 DNA 分子及其编码的多肽。这些 DNA 分子可作为启动子或蛋白质编码序列或 UTR 或 3'终止序列，用于指定细胞中的基因产物，也可用于控制染色体中基因的行为，控制基因的表达，或作为基因绘图、识别或分离相同或相关 DNA 片段、或识别特定生物个体或具有共同性状的生物群体的工具。
• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Methods of expressing products in mammalian cells
  申请人：Intrexon CEU, INC.

  公开号：US10000770B2

  公开（公告）日：2018-06-19

  The invention relates to newly identified selectable marker systems, cells for use in a selectable marker system, and methods for using the selectable marker systems.

  本发明涉及新发现的可选择标记系统、用于可选择标记系统的细胞以及使用可选择标记系统的方法。
• Ureohydrolases as dominant selectable markers in yeast
  申请人：HEINEKEN SUPPLY CHAIN B.V.

  公开号：US10301633B2

  公开（公告）日：2019-05-28

  The invention relates to a nucleic acid molecule encoding a novel selection marker. Said marker is a guanidinobutyrase from Kluyveromyces lactis, which, when expressed in Saccharomyces, allows the growth of the yeast in the presence of guanidinobutyrate as the sole nitrogen source. Said marker can be used in a method for producing a microorganism having an altered genome. The invention further relates to a set of constructs, comprising a first construct comprising a recognition site for an endonuclease, a first region of homology with a target gene of a microorganism, and a first part of a nucleotide sequence encoding the selection marker, and a second construct comprising a second part of the nucleotide sequence encoding the selection marker, a second region of homology with the target gene of the microorganism, and a copy of the endonuclease recognition site. The invention further relates to methods for altering a target gene in a microorganism, to methods for producing a microorganism, and to microorganisms that are produced by the methods of the invention.

  本发明涉及一种编码新型选择标记的核酸分子。所述标记是一种来自乳酸克鲁维酵母菌的鸟苷酸丁酸酶，当在酵母中表达时，可使酵母在鸟苷酸丁酸作为唯一氮源的情况下生长。所述标记可用于生产具有改变基因组的微生物的方法中。本发明进一步涉及一组构建体，包括第一构建体，该构建体包含内切酶的识别位点、与微生物靶基因同源的第一区域和编码选择标记的核苷酸序列的第一部分；以及第二构建体，该构建体包含编码选择标记的核苷酸序列的第二部分、与微生物靶基因同源的第二区域和内切酶识别位点的拷贝。本发明还涉及改变微生物中目的基因的方法、生产微生物的方法以及用本发明方法生产的微生物。