| 946387-81-1

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• CAS: 946387-81-1
• 化学式: C₂₇H₂₅N₅O₅
• 分子量: 499.526
• InChiKey: YOPLTQVLWQYWAO-UHFFFAOYSA-N

反应信息

• 作为反应物：
  描述：

  、 以 水 、 乙腈 为溶剂， 反应 3.0h， 以44%的产率得到
  参考文献：
  名称：

  Fluorescent Saxitoxins for Live Cell Imaging of Single Voltage-Gated Sodium Ion Channels beyond the Optical Diffraction Limit

  摘要：

  A desire to better understand the role of voltage-gated sodium channels (Nays) in signal conduction and their dysregulation in specific disease states motivates the development of high precision tools for their study. Nature has evolved a collection of small molecule agents, including the shellfish poison (+)-saxitoxin, that bind to the extracellular pore of select Na-v isoforms. As described in this report, de novo chemical synthesis has enabled the preparation of fluorescently labeled derivatives of (+)-saxitoxin, STX-Cy5, and STX-DCDHF, which display reversible binding to Nays in live cells. Electrophysiology and confocal fluorescence microscopy studies confirm that these SIX-based dyes function as potent and selective Nay labels. The utility of these probes is underscored in single-molecule and super-resolution imaging experiments, which reveal Nay distributions well beyond the optical diffraction limit in subcellular features such as neuritic spines and filopodia.

  DOI：

  10.1016/j.chembiol.2012.05.021