5-Amino-2-nitrobenzamid | 73536-37-5

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 英文名称: 5-Amino-2-nitrobenzamid
• 英文别名: 5-amino-2-nitrobenzoic acid amide；ANB amide；ANB-NH2；5-Amino-2-nitrobenzamide
• CAS: 73536-37-5
• 化学式: C₇H₇N₃O₃
• 分子量: 181.151
• InChiKey: CCEJTEOJAKTTFU-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.2
• 重原子数：
  13
• 可旋转键数：
  1
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  115
• 氢给体数：
  2
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  5-Azido-2-nitrobenzamid 以7%的产率得到
  参考文献：
  名称：

  NAKAYAMA HITOSHI; NOZAWA MASAYUKI; KANAOKA YUICHI, CHEM. AND PHARM. BULL., 1979, 27, NO 11, 2775-2780

  摘要：

  DOI：

文献信息

• Highly Specific Substrates of Proteinase 3 Containing 3-(2-Benzoxazol-5-yl)-<scp>l</scp>-alanine and Their Application for Detection of This Enzyme in Human Serum
  作者：Magdalena Wysocka、Adam Lesner、Katarzyna Guzow、Julia Kulczycka、Anna Łȩgowska、Wiesław Wiczk、Krzysztof Rolka

  DOI：10.1021/ac1004103

  日期：2010.5.1

  A set of benzoxazolyl-l-alanine derivates along with the MCA moiety (donors of fluorescence) were introduced into a proteinase 3 (PR3) substrate with a C-terminal ANB-NH2 that serves as a fluorescence acceptor. Five substrates with general formula X-Tyr-Tyr-Abu-ANB-NH2 were synthesized, and their kinetic parameters against proteinase 3 were determined. The highest kcat/KM value, 1.5 × 106 M−1 s−1, was obtained for (Pyr)Box-Ala-Tyr-Tyr-Abu-NH2 where (Pyr)Box-Ala stands for N-methylpyrrole benzoxazole-l-alanine. Titration of this peptide with proteinase 3 resulted in measurable fluorescence at an enzyme amount equal to 29 pmol. This substrate was selected used to detect quantifiable levels of proteinase 3 in serum samples, including those of normal subjects. For all c-ANCA-positive samples (diagnosed Wegener granulomatosis), a significant increase of PR3 concentration was observed. Wegener granulomatosis is a severe autoimmune disease causing inflammation of the blood vessels. Our results clearly show that this substrate can be used for the construction of a very reliable, inexpensive, and easy to use diagnostic test for PR3 determination.

  一组苯并噁唑基-l-丙氨酸衍生物与MCA基团（荧光供体）被引入到具有C端ANB-NH2的蛋白酶3（PR3）底物中，该底物作为荧光受体。合成了五种一般式为X-Tyr-Tyr-Abu-ANB-NH2的底物，并确定了它们对蛋白酶3的动力学参数。得到的最高kcat/KM值为1.5 × 10^6 M−1 s−1，出现在(Pyr)Box-Ala-Tyr-Tyr-Abu-NH2中，其中(Pyr)Box-Ala代表N-甲基吡咯烷苯并噁唑-l-丙氨酸。用蛋白酶3对该肽进行滴定，在酶量为29 pmol时观察到了可测量的荧光。选择该底物用于检测血清样本中蛋白酶3的定量水平，包括正常受试者的样本。对于所有c-ANCA阳性样本（诊断为韦格纳肉芽肿病），观察到PR3浓度显著增加。韦格纳肉芽肿病是一种严重的自身免疫疾病，会导致血管炎症。我们的结果清楚地表明，这种底物可以用于构建一种非常可靠、廉价且易于使用的PR3检测诊断测试。
• Renin-inhibitory oligopeptides, their preparation and use
  申请人：SANKYO COMPANY LIMITED

  公开号：EP0228192A2

  公开（公告）日：1987-07-08

  Oligopeptides of formula (I): where R1R7 are various organic groups and n and m are 0 or 1, have renin-inhibitory activity and are particularly suitable for oral administration. They may be prepared by condensing their component amino acids or lower oligopeptides using conventional peptide synthesis reactions.

  式(I)的寡肽： 其中 R1R7 为各种有机基团，n 和 m 为 0 或 1，具有肾素抑制活性，特别适合口服。它们可以通过传统的肽合成反应缩合其氨基酸组分或低级寡肽来制备。
• Tetrakisazo compound and ink containing the compound
  申请人：SUMITOMO CHEMICAL COMPANY, LIMITED

  公开号：EP0645436A1

  公开（公告）日：1995-03-29

  A tetrakisazo compound represented by the following formula (1) in the free acid form: wherein Q₁ and Q₂ independently represent a phenylene or naphthylene group which may be optionally substituted, R₄, R₅ and R₆ independently represent a hydrogen atom, a halogen atom, a hydroxy group, a sulfonic acid group or an amino, alkyl, alkoxy, carbamoyl or sulfamoyl group which may be optionally substituted, m is 0 or 1 and n is 1 or 2, and an ink which comprises the tetrakisazo compound. The ink exhibit excellent properties for ink jet printing.

  游离酸形式的下式（1）所代表的四异氮化合物： 其中，Q₁ 和 Q₂ 各自代表可被任选取代的亚苯基或亚萘基，R₄、R₅ 和 R₆ 各自代表氢原子、卤素原子、羟基、磺酸基或可被任选取代的氨基、烷基、烷氧基、氨基甲酰基或氨基磺酰基，m 为 0 或 1，n 为 1 或 2。这种油墨在喷墨打印方面具有优异的性能。
• Monoazoverbindungen enthaltend mindestens eine Carbonamidgruppe
  申请人：CIBA-GEIGY AG

  公开号：EP0162806B1

  公开（公告）日：1989-01-25
• US4737581A
  申请人：——

  公开号：US4737581A

  公开（公告）日：1988-04-12