1,3-dimethyl-1,6,7,8,-tetrahydropyrido<1,2e>purine-2,4,9-<3H>trione | 59045-81-7

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 咪唑并嘧啶类
• 英文名称: 1,3-dimethyl-1,6,7,8,-tetrahydropyrido<1,2e>purine-2,4,9-<3H>trione
• 英文别名: 1,3-dimethyl-1,6,7,8-tetrahydropyrido[1,2-e]purine-2,4,9(3H)-trione；1,3-dimethyl-7,8-dihydro-6H-purino[9,8-a]pyridine-2,4,9-trione
• CAS: 59045-81-7
• 化学式: C₁₁H₁₂N₄O₃
• 分子量: 248.241
• InChiKey: PRFAYKIWAUAMJC-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.2
• 重原子数：
  18
• 可旋转键数：
  0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.45
• 拓扑面积：
  75.5
• 氢给体数：
  0
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  1,3-dimethyl-1,6,7,8,-tetrahydropyrido<1,2e>purine-2,4,9-<3H>trione 、 N6-(6-氨基己基)-黄素腺嘌呤二核苷酸 以 二甲基亚砜 为溶剂， 以53%的产率得到
  参考文献：
  名称：

  黄素腺嘌呤二核苷酸作为均相比色免疫测定中的标记。

  摘要：

  DOI：

  10.1021/ac00227a021

文献信息

• Specific binding assay with a prosthetic group as a label component
  申请人：Miles Laboratories, Inc.

  公开号：US04238565A1

  公开（公告）日：1980-12-09

  A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate. Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising such prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after the binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group-labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.

  一种用有机假体残基作为标签成分的特定结合测定法，用于确定液体介质中的配体，例如黄素腺嘌呤二核苷酸、黄素单核苷酸或血红素残基。优选地，标签成分是仅包含假体残基的全酶残基或是以全酶复合物形式将该假体残基与缺酶形式的酶结合而成的全酶残基。在前一种情况下，标签成分优选地通过在结合反应启动后添加缺酶并测量所得的全酶活性来监测测定。在后一种情况下，标签成分仅通过测量全酶活性来监测。测定方法可以遵循传统的同质和异质方案。用于测定的优选缺酶包括缺葡萄糖氧化酶和缺过氧化物酶。该测定法具有色度读取和易于自动化技术适应的优点。还披露了用于测定的假体标记和酶标记的结合物，以及制备后者结合物的方法。
• FMN-Labeled specific binding assay
  申请人：Miles Laboratories, Inc.

  公开号：US04318982A1

  公开（公告）日：1982-03-09

  A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate. Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising such prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after the binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group-labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.

  一种特定的结合测定法，用于确定液态介质中的配体，采用有机假体基残基作为标记物组分的标记共轭物。偏好地，标记组分是仅为假体基残基或为包含这样的假体基残基与一个无假体酶的蛋白质组成的全酶残基的全酶复合物。在前一种情况下，最好通过在结合反应开始后添加无假体酶并测量所得到的全酶活性来监测测定中的标记组分。在后一种情况下，标记组分仅通过测量全酶活性来监测。测定方法可以遵循传统的均相和非均相方案。用于测定的首选无假体酶是无葡萄糖氧化酶和无过氧化物酶。该测定方法具有色度读数和易于适应自动化技术的优点。还公开了用于测定的假体基标记和酶标记共轭物，以及制备后者共轭物的方法。
• Fad-labeled specific binding assay monitored with apoglutathione
  申请人：Miles Laboratories, Inc.

  公开号：US04318983A1

  公开（公告）日：1982-03-09

  A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate. Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising such prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after the binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group-labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.

  一种特定的结合测定法，用于确定液体介质中的配体，采用有机假体残基作为标签组分的标记共轭物，例如黄素腺嘌呤二核苷酸残基，黄素单核苷酸或血红素。优选地，标签组分仅为假体残基或是由假体残基与裸酶组成的全酶残基，形成全酶复合物。在前一种情况下，通过在结合反应启动后添加裸酶并测量所得的全酶活性来监测测定中的标签组分。在后一种情况下，标签组分仅通过测量全酶活性来监测。该测定方法可以遵循传统的均相和非均相方案。用于测定的优选裸酶是裸葡萄糖氧化酶和裸过氧化物酶。该测定具有色度读出和易于自动化技术的优点。还公开了用于测定的假体标记和酶标记共轭物，以及制备后者共轭物的方法。
• Heme-labeled specific binding assay
  申请人：Miles Laboratories, Inc.

  公开号：US04340668A1

  公开（公告）日：1982-07-20

  A specific binding assay for determining a ligand in a liquid medium employing an organic prosthetic group residue, such as a residue of flavin adenine dinucleotide, flavin mononucleotide, or heme, as a label component in the labeled conjugate. Preferably, the label component is the prosthetic group residue alone or is a holoenzyme residue comprising such prosthetic group residue combined with an apoenzyme in the form of a holoenzyme complex. In the former case, the label component preferably is monitored in the assay by adding an apoenzyme after the binding reaction has been initiated and measuring the resultant holoenzyme activity. In the latter case, the label component is monitored simply by measuring holoenzyme activity. The assay method may follow conventional homogeneous and heterogeneous schemes. Preferred apoenzymes for use in the assay are apoglucose oxidase and apoperoxidase. The assay offers the advantages of colorimetric read-out and of being readily adaptable to automated techniques. Also disclosed are prosthetic group-labeled and enzyme-labeled conjugates for use in the assay, as well as a method for preparing the latter conjugates.

  一种特定的结合测定法，用于在液态介质中确定配体，利用有机假体基残基作为标记组分的标记共轭物，例如黄素腺嘌呤二核苷酸、黄素单核苷酸或血红素残基。优选地，标记组分是假体基残基单独存在或是由这样的假体基残基与一个原酶组成的全酶残基组合而成的全酶复合物。在前一种情况下，标记组分最好是在结合反应开始后加入原酶，并测量所得到的全酶活性来监测结合反应。在后一种情况下，标记组分仅通过测量全酶活性来监测。测定方法可以遵循常规的均相和非均相方案。用于测定的优选原酶是去葡萄糖氧化酶和去过氧化酶。该测定方法具有色度读出和易于自动化技术适应的优点。还公开了用于测定的假体基标记和酶标记共轭物，以及制备后者共轭物的方法。
• US4255566A
  申请人：——

  公开号：US4255566A

  公开（公告）日：1981-03-10