4-alpha-glucanotransferase | 9032-09-1

• 分子结构分类: 转移酶 - 糖基转移酶 - 己糖基转移酶
• 英文名称: 4-alpha-glucanotransferase
• 英文别名: disproportionating enzyme；dextrin glycosyltransferase；D-enzyme；debranching enzyme maltodextrin glycosyltransferase；amylomaltase；dextrin transglycosylase；1,4-alpha-D-glucan:1,4-alpha-D-glucan 4-alpha-D-glycosyltransferase；cyclodextrin-α-1,4-glucanotransferase
• CAS: 9032-09-1

反应信息

• 作为试剂：
  描述：

  2-Deoxy-D-glucose 、 α-环糊精 在 4-alpha-glucanotransferase sodium azide 、 三羟甲基氨基甲烷 、 calcium chloride 作用下， 以 水 为溶剂， 反应 2.5h， 以19%的产率得到2-deoxy-maltose
  参考文献：
  名称：

  Specificity of amylases and cyclodextrin-glucanotransferase in reactions with 2-deoxy-maltooligosaccharides

  摘要：

  2-Deoxy-maltooligosaccharides of different chain length were tested as substrates for exoand endo-amylases. Cleavage occurred with beta-amylase, yielding 2,2'-dideoxy-maltose, and with amyloglucosidase. With the alpha-amylase from Thermomonospora curvata tris-(2-deoxy)-maltotriose and the corresponding tetra- and pentasaccharides were formed. Porcine pancreatic a-amylase did not tolerate the deoxygenated substrate, nor were cyclization experiments with cyclodextrin-glucanotransferase (CGT) successful. In a coupling reaction with CGT, however, a series of transfer products to the acceptor 2-deoxyglucose were obtained. (C) 1997 Elsevier Science Ltd.

  DOI：

  10.1016/s0008-6215(97)00040-2