1-(2-硝基苯基)乙基戊-4-烯酸酯 | 264258-90-4

• 分子结构分类: 有机化合物 - 苯类化合物 - 苯和取代衍生物
• 中文名称: 1-(2-硝基苯基)乙基戊-4-烯酸酯
• 英文名称: 1-(2-nitrophenyl)ethyl 4-pentenoate
• 英文别名: 1-(2-Nitrophenyl)ethyl pent-4-enoate
• CAS: 264258-90-4
• 化学式: C₁₃H₁₅NO₄
• 分子量: 249.266
• InChiKey: HZPVGGVXRAMHEW-UHFFFAOYSA-N

物化性质

• 沸点：
  344.2±22.0 °C(Predicted)
• 密度：
  1.154±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  2.9
• 重原子数：
  18
• 可旋转键数：
  6
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.31
• 拓扑面积：
  72.1
• 氢给体数：
  0
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  1-(2-硝基苯基)乙基戊-4-烯酸酯 在 dihydrogen hexachloroplatinate 三氯硅烷 作用下， 生成 1-(2-nitrophenyl)ethyl 5-trichlorosilylpentanoate
  参考文献：
  名称：

  Photoactivation of a Substrate for Cell Adhesion under Standard Fluorescence Microscopes

  摘要：

  Cell-culturing substrates where cell adhesion can be switched on by external stimuli during cell cultivation are useful scaffolds for tissue engineering, cell-based drug screening, and fundamental cellular studies. Here, we show a new strategy for photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. A glass substrate chemically modified with an alkylsiloxane having a photocleavable 2-nitrobenzyl group was coated with bovine serum albumin to prevent cell adhesion. Upon irradiation under a fluorescence microscope, the protein was replaced with fibronectin, which made the irradiated region cell-adhesive. Subsequent seeding of HEK293 or COS7 cells produced patterns corresponding to the irradiated patterns. We succeeded for the first time in positioning single cells in proximity to cultivating single cells. The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions.

  DOI：

  10.1021/ja044684c
• 作为产物：
  描述：

  4-戊烯酸 、 1-(2-硝基苯基)乙醇 在 4-二甲氨基吡啶 、 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 作用下， 生成 1-(2-硝基苯基)乙基戊-4-烯酸酯
  参考文献：
  名称：

  Photoactivation of a Substrate for Cell Adhesion under Standard Fluorescence Microscopes

  摘要：

  Cell-culturing substrates where cell adhesion can be switched on by external stimuli during cell cultivation are useful scaffolds for tissue engineering, cell-based drug screening, and fundamental cellular studies. Here, we show a new strategy for photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. A glass substrate chemically modified with an alkylsiloxane having a photocleavable 2-nitrobenzyl group was coated with bovine serum albumin to prevent cell adhesion. Upon irradiation under a fluorescence microscope, the protein was replaced with fibronectin, which made the irradiated region cell-adhesive. Subsequent seeding of HEK293 or COS7 cells produced patterns corresponding to the irradiated patterns. We succeeded for the first time in positioning single cells in proximity to cultivating single cells. The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions.

  DOI：

  10.1021/ja044684c

文献信息

• Novel Silane Coupling Agents Containing a Photolabile 2-Nitrobenzyl Ester for Introduction of a Carboxy Group on the Surface of Silica Gel
  作者：Kazuo Yamaguchi、Takashi Kitabatake、Masashi Izawa、Tomomichi Fujiwara、Hiroki Nishimura、Tatsuhiro Futami

  DOI：10.1246/cl.2000.228

  日期：2000.3

  Silane coupling agents containing a carboxy group are not known because the chloro- or alkoxy group is reactive to the carboxylic acid. In this study, silane coupling agents bearing a 2-nitrobenzyl ester as a protecting group of carboxylic acid were synthesized and applied for surface modification of silica gel followed by uv irradiation under neutral conditions to generate carboxy groups on the surface

  含羧基的硅烷偶联剂是未知的，因为氯或烷氧基对羧酸具有反应性。在本研究中，合成了带有 2-硝基苄酯作为羧酸保护基团的硅烷偶联剂，并将其应用于硅胶的表面改性，然后在中性条件下进行紫外线照射以在表面生成羧基。
• Photoactivation of a Substrate for Cell Adhesion under Standard Fluorescence Microscopes
  作者：Jun Nakanishi、Yukiko Kikuchi、Tohru Takarada、Hidekazu Nakayama、Kazuo Yamaguchi、Mizuo Maeda

  DOI：10.1021/ja044684c

  日期：2004.12.1

  Cell-culturing substrates where cell adhesion can be switched on by external stimuli during cell cultivation are useful scaffolds for tissue engineering, cell-based drug screening, and fundamental cellular studies. Here, we show a new strategy for photoactivation of a substrate for cell adhesion under standard fluorescence microscopes. A glass substrate chemically modified with an alkylsiloxane having a photocleavable 2-nitrobenzyl group was coated with bovine serum albumin to prevent cell adhesion. Upon irradiation under a fluorescence microscope, the protein was replaced with fibronectin, which made the irradiated region cell-adhesive. Subsequent seeding of HEK293 or COS7 cells produced patterns corresponding to the irradiated patterns. We succeeded for the first time in positioning single cells in proximity to cultivating single cells. The present method provides a general strategy for positioning single cells of same or different types at any locations on the substrate and will be useful for studying cell-cell interactions.