METHOD OF PLASTID TRANSFORMATION IN ASTERACEAE, VECTOR FOR USE THEREIN AND PLANTS THUS OBTAINED
申请人:LELIVELT Cecilia Lucia Clara
公开号:US20100218277A1
公开(公告)日:2010-08-26
The present invention relates to a method for the transformation of plastid genomes of plant species, in particular Asteraceae plant species, comprising the steps of providing a transformation vector carrying a DNA sequence of interest; subjecting a plant material, which comprises plastids, to a transformation treatment in order to allow the plastids to receive the transformation vector; placing the thus treated plant material for a period of time into contact with a culture medium without selection agent; subsequently placing the plant material into contact with a culture medium comprising a selection agent; and refreshing the culture medium comprising a selection agent to allow plant material comprising plastids that have acquired the DNA of interest to grow into transformants.
本发明涉及一种植物种类(特别是菊科植物种类)质体基因组转化的方法,包括以下步骤:提供携带感兴趣的DNA序列的转化载体;将包含质体的植物材料进行转化处理,以使质体接受转化载体;将经过处理的植物材料放置在不含选择剂的培养基中一段时间;随后将植物材料放置在含有选择剂的培养基中;并更新含有选择剂的培养基,以使包含已获得感兴趣DNA的质体的植物材料生长成转化体。