Neighboring-group participation in organic redox reactions: effect of tertiary amine and pyridine groups on the kinetics and mechanism of thioether-sulfoxide interconversions
EHLKINSON, R. S.;EREMEEV, A. V.;MISHNEV, A. F.;BLEJDELIS, YA. YA.;SEMENIX+, XIMIYA GETEROTSIKL. SOEDIN., 1985, N 1, 53-58
作者:EHLKINSON, R. S.、EREMEEV, A. V.、MISHNEV, A. F.、BLEJDELIS, YA. YA.、SEMENIX+
DOI:——
日期:——
METHOD FOR PREPARING FUSION PROTEIN BY TRANS-SPLICING METHOD
申请人:Kawakami Masayuki
公开号:US20080187941A1
公开(公告)日:2008-08-07
An object to be achieved by the present invention is to provide a method that enables convenient production of a target fusion protein within a short time without constructing any expression vector. The present invention provides a method for producing a fusion protein comprising a first protein and a second protein, which comprises the steps of: introducing a nucleic acid molecule (pre-trans-splicing molecule) or a vector capable of expressing such a nucleic acid molecule into a cell expressing the first protein, wherein the nucleic acid molecule contains a gene sequence encoding the second protein and a sequence which is capable of inducing trans-splicing through binding to pre-mRNA that is generated from a region containing a gene sequence that encodes the first protein; and recovering a fusion protein comprising the first protein and the second protein generated within the cell.
Schneider, Justus Liebigs Annalen der Chemie, 1912, vol. 386, p. 340