7-(diethylamino)-2-oxo-N-((pyridin-2-yl)methyl)-2H-chromene-3-carboxamide | 1117894-52-6

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 香豆素及其衍生物
• 英文名称: 7-(diethylamino)-2-oxo-N-((pyridin-2-yl)methyl)-2H-chromene-3-carboxamide
• 英文别名: 7-(diethylamino)-2-oxo-N-(pyridin-2-ylmethyl)chromene-3-carboxamide
• CAS: 1117894-52-6
• 化学式: C₂₀H₂₁N₃O₃
• 分子量: 351.405
• InChiKey: QOIBJRUPFJUTDP-UHFFFAOYSA-N

物化性质

• 熔点：
  170 °C
• 沸点：
  638.4±55.0 °C(predicted)
• 密度：
  1.254±0.06 g/cm3(Temp: 20 °C; Press: 760 Torr)(predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  3.3
• 重原子数：
  26
• 可旋转键数：
  6
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.25
• 拓扑面积：
  71.5
• 氢给体数：
  1
• 氢受体数：
  5

反应信息

• 作为产物：
  描述：

  2-氨甲基吡啶 、 7-(二乙基氨基)香豆素-3-羧酸 在 4-二甲氨基吡啶 、 N,N'-二环己基碳二亚胺 作用下， 以 氯仿 为溶剂， 反应 6.0h， 以60%的产率得到7-(diethylamino)-2-oxo-N-((pyridin-2-yl)methyl)-2H-chromene-3-carboxamide
  参考文献：
  名称：

  Coumarin-Derived Cu²⁺-Selective Fluorescence Sensor: Synthesis, Mechanisms, and Applications in Living Cells

  摘要：

  A novel coumarin-based fluorogenic probe bearing the 2-picolyl unit (1) was developed as a fluorescent chemosensor with high selectivity and suitable affinity in biological systems toward Cu2+ over other cations tested. The fluorescence on-off mechanism was studied by femtosecond time-resolved fluorescence (TRF) upconversion technique and ab initio calculations. The receptor can be applied to the monitoring of Cu2+ ion in aqueous solution with a pH span 4-10. To confirm the suitability of 1 for biological applications, we also employed it for the fluorescence detection of the changes of intracellular Cu2+ in cultured cells. The results indicate that 1 should be useful for the fluorescence microscopic imaging and the study on the biological functions Of Cu2+..

  DOI：

  10.1021/ja808611d

文献信息

• Method and kit for determination of free copper in serum
  申请人：IGEA RESEARCH CORPORATION

  公开号：US10663458B2

  公开（公告）日：2020-05-26

  The present invention relates to a new method for the determination of free copper in serum. In particular to a method with a high degree of sensitivity and accuracy for the determination of free copper in serum samples of patients with Alzheimer's disease and Wilson's disease and other forms of free copper dyshomeostasis. The invention also relates to kits for the determination of free copper in serum comprising filter devices and coumarin fluorescent probe.

  本发明涉及一种测定血清中游离铜的新方法。特别是一种具有高灵敏度和准确性的方法，用于测定阿尔茨海默氏症和威尔逊氏病以及其他形式的游离铜失衡患者血清样本中的游离铜。本发明还涉及由过滤装置和香豆素荧光探针组成的测定血清中游离铜的试剂盒。
• Composition for enzyme immunoassay using immunofluorescence and uses thereof
  申请人：BIOMERIEUX

  公开号：US10605803B2

  公开（公告）日：2020-03-31

  A composition for enzyme immunoassay using immunofluorescence comprises (i) a fluorogenic enzymatic substrate and (ii) a quenched fluorogenic compound that forms a fluorescent compound after hydrolysis of the fluorogenic enzymatic substrate. The composition may be useful for performing a method for in vitro detection and/or quantification of an analyte of a liquid test sample liable to contain the analyte.

  一种利用免疫荧光法进行酶免疫测定的组合物包括：(i) 含氟酶底物；(ii) 在含氟酶底物水解后形成荧光化合物的淬灭含氟化合物。该组合物可用于对可能含有被分析物的液体测试样品进行体外检测和/或定量的方法。
• METHOD AND KIT FOR DETERMINATION OF FREE COPPER IN SERUM
  申请人：Canox4drug S.p.A.

  公开号：EP2917739B1

  公开（公告）日：2018-02-21
• METHOD FOR DETERMINING FREE COPPER
  申请人：Canox4drug S.p.A.

  公开号：EP2992326A2

  公开（公告）日：2016-03-09
• COMPOSITION POUR DOSAGE IMMUNO-ENZYMATIQUE PAR IMMUNOFLUORESCENCE ET SES UTILISATIONS
  申请人：Biomérieux

  公开号：EP3207371A1

  公开（公告）日：2017-08-23