Ferroptosis is an iron-mediated, caspase-independent pathway of cell death that is accompanied with the accumulations of reactive oxygen species (ROS) and oxygenases, as well as being involved in many other pathophysiological procedures. However, specific and rapid monitoring of ferroptosis in living cells or tissues has not been achieved so far. Herein, a quinoxalinone-based fluorescent probe (termed as Quinos-4, or QS-4) with a reactive aromatic thioether moiety was designed for ferroptosis identification. Upon exposing it to high levels of ROS and hemeoxygenase-1 (HO-1), which are considered as the biochemical characteristics of ferroptosis, QS-4 could be oxidized into a sulfoxide derivative (QSO-4) and its original aggregation-induced enhanced red fluorescence emission could be converted to green fluorescence emission sharply. On the basis of this unique reaction-induced color conversion, this molecular probe can be employed for identifying the occurrence of ferroptosis both in vitro and in vivo.
铁死亡是一种
铁介导的、依赖于半胱天冬酶的
细胞死亡途径,伴随着活性氧(ROS)和氧酶的积累,并参与许多其他病理生理过程。然而,迄今为止尚未实现对活细胞或组织中
铁死亡的特异性和快速监测。在此,我们设计了一种基于
喹喔啉酮的荧光探针(称为QuinOS-4,或QS-4),它具有反应性的芳香
硫醚部分,用于
铁死亡的识别。在暴露于高
水平的ROS和血红素氧合酶-1(HO-1),这些被认为是
铁死亡的生化特征时,QS-4可以被氧化成亚砜衍
生物(QSO-4),并且其原有的聚集诱导增强的红色荧光发射可以迅速转换为绿色荧光发射。基于这种独特的反应诱导颜色转换,这种分子探针可以用于在体外和体内识别
铁死亡的发生。